RNase Inhibitor Hu

126193 RT35 Media/Uploads/RT35-020(13).webp RNase Inhibitor Hu RNase Inhibitor Hu <p><span style="color: rgb(89, 89, 89); font-size: 16px; font-family: Tahoma; letter-spacing: 0.25px;">Supplied at a concentration of 40 U/µL. (Storage temperature: -20°C without a defrost cycle)</span></p><p><span style="color: rgb(89, 89, 89); font-size: 16px; font-family: Tahoma; letter-spacing: 0.4px;">The RNase Inhibitor Hu is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.</span></p><h4 _ngcontent-serverapp-c2480474117="" class="section" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 28px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; font-variant: normal; border: 0px; letter-spacing: 0px;"><span style="font-size: 16px;"><span style="font-family: Tahoma;">Features</span></span></h4><p><span style="font-size: 16px; font-family: Tahoma;"><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-onedatalayer-source="Product Details" class="ng-star-inserted" style="color: rgb(89, 89, 89); background-color: rgb(255, 255, 255); letter-spacing: 0.25px;"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details></span></p><section _ngcontent-serverapp-c2480474117="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c2480474117="" class="description-box" lg-uid="lg0" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c2480474117="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border-style: none; border-width: 0px; letter-spacing: 0.5px;"><ul style="width: calc(66.6667% - 12px); padding: 0px 0px 0px 18px; border: 0px; list-style-image: initial;"><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Heat stable noncompetitive inhibitor of pancreatic-type ribonucleases e.g., RNases A, B and C</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Active at 60°C and retains full stability at 37°C for at least 4 weeks with no loss of activity</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Active in diverse reaction conditions and in various buffers&nbsp;</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Preserves RNA without inhibiting other RNases and modifying enzymes</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Recombinant protein expressed in <em style="padding: 0px; border: 0px;">E.coli</em> carrying the human placental protein gene</li></ul></div></section><section _ngcontent-serverapp-c3508226301="" scrollanchor="product-details" style="color: rgb(89, 89, 89); padding: 0px; font-size: 16px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; border: 0px; letter-spacing: 0.25px;"><span style="font-family: Tahoma;"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></span><h4 _ngcontent-serverapp-c2480474117="" class="intro" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 40px; border: 0px;"><span style="font-size: 16px;"><span style="font-family: Tahoma;">Product Details</span></span></h4><span style="font-family: Tahoma;"><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-navigation="Product Details" data-onedatalayer-source="Product Details" class="ng-star-inserted"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"><section _ngcontent-serverapp-c2480474117="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c2480474117="" class="description-box" lg-uid="lg1" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c2480474117="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border-style: none; border-width: 0px; letter-spacing: 0.5px;"><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;">RNase Inhibitor Hu is a thermally resistant, 50 kDa recombinant human placental protein expressed in E<em style="padding: 0px; border: 0px;">scherichia coli</em> that inhibits RNase activity of common eukaryotic enzymes such as RNase A, RNase B, RNase C.</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;">Native ribonuclease inhibitor is a large (~450 residues, ~50 kDa), acidic (pI ~4.7), leucine-rich repeat protein. Ribonuclease inhibitor shows remarkable affinity for pancreatic-type ribonucleases and acts as a potent noncompetitive inhibitor of these enzymes. The resulting noncovalent complexes are some of the tightest known in biology. Ribonuclease inhibitor does not interfere with the activities of other RNases and modifying enzymes like polymerases and reverse transcriptases.</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;">Ribonuclease inhibitors are essential in molecular biology to minimize the action of ubiquitous ribonucleases that can compromise RNA experiments. The source of RNases within skin, air or labware is primarily microorganisms: bacteria, viruses and fungi.</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;">Benefits of using RNase Inhibitor Human</p><ul style="width: calc(66.6667% - 12px); padding: 0px 0px 0px 18px; border: 0px; list-style-image: initial;"><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Recombinant human enzyme is thermostable and active up to 60°C</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Maximum protection of RNA integrity for intact RNA during handling, storage and assays</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Outperforms alternative supplier products, consistently achieving high RNA integrity</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Suitable for higher temperature applications in RT-PCR, RT-qPCR and RT-LAMP</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Greater resistance to oxidation with more activity at low reducing conditions</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Active over a broad pH range (5.5 to 9.0) and in various buffers</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Improved yields in transcription/translation in in vitro transcription and translation (IVT) systems</li></ul><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;">RNase Inhibitor Hu is a heat stable recombinant human placental protein expressed in E.coli. RNase Inhibitor Hu retains full stability at 37°C for at least 4 weeks with no loss of activity.&nbsp;</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;">RNase Inhibitor Hu is supplied with 20 mM Hepes-KOH (pH 7.6), 50 mM KCl, 8 mM reducing agent, 50% (v/v) glycerol. The enzyme is active up to 60°C and at a minimum 0.5–1 mM DTT concentration ranges.</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;">One unit is defined as the amount of enzyme required to inhibit the activity of 5 ng RNase A by 50%.</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;">In applications where RNase inhibitor activity at higher temperatures is less critical, <a href="https://www.qiagen.com/us/products/discovery-and-translational-research/enzymes-for-molecular-biology/rnase-inhibitor" style="color: rgb(0, 77, 159); padding: 0px; border: 0px;">RNase Inhibitor</a> Y9240L is stable up to 50°C and is suitable for use at temperatures ≤50°C.&nbsp;</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;"><br></p></div></section></wca-product-description></wca-product-details><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-onedatalayer-source="Product Details" class="ng-star-inserted"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details></span><h4 _ngcontent-serverapp-c2480474117="" class="section" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 28px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; font-variant: normal; border: 0px; letter-spacing: 0px;"><span style="font-size: 16px;"><span style="font-family: Tahoma;">Performance</span></span></h4><span style="font-family: Tahoma;"><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-onedatalayer-source="Product Details" class="ng-star-inserted"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"><section _ngcontent-serverapp-c2480474117="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c2480474117="" class="description-box" lg-uid="lg2" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c2480474117="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border-style: none; border-width: 0px; letter-spacing: 0.5px;"><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;">RNase Inhibitor Hu outperforms alternative supplier products, consistently achieving high RNA integrity (see figure “<a title="Summary of RNA integrity number (RIN) results for three RNase Inhibitors" href="https://www.qiagen.com/binary/resource/BC_1532_ICS_OEM_QPRO_2398_RNase_inhibitor/1_5_WebFullSizeImage.jpg" data-image-pimid="245394" class="lightbox-link" data-sub-html="&lt;div class=&quot;image-caption-container&quot;&gt;&lt;h3 class=&quot;image-caption-heading&quot;&gt;Summary of RNA integrity number (RIN) results for three RNase Inhibitors&lt;/h3&gt; &lt;div class=&quot;image-caption-content&quot;&gt;Two quantities, 20 and 40 U, of each RNase inhibitor were added per 20 μL reaction. The RIN was determined to establish the RNA protection level. RNase Inhibitor Hu consistently exhibited the highest RIN. Points: mean, whiskers – SD. *, # – determination for statistically significant differences between RNase Inhibitor Hu and RNase inhibitors from Suppliers X and Y, respectively. *, #: p &lt;0.05; ****, ####: p &lt;0.0001: Student‘s t-test.&lt;/div&gt;&lt;/div&gt;" data-src="null" style="color: rgb(0, 77, 159); padding: 0px; border: 0px;"> <span style="padding: 0px; border: 0px; letter-spacing: inherit;">Summary of RNA integrity number (RIN) results for three RNase Inhibitors</span></a>”).</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;">RNase Inhibitor Hu displays full stability at 37°C for at least 4 weeks. High thermostability guarantees high activity even after exposure to increased temperatures e.g., during worktime on the bench or demanding shipping conditions (see figure “<a title="Stability of RNase Inhibitor Hu at 37°C over a 6-week period" href="https://www.qiagen.com/binary/resource/BC_1530_ICS_OEM_QPRO_2398_RNase_inhibitor/1_5_WebFullSizeImage.jpg" data-image-pimid="245402" class="lightbox-link" data-sub-html="&lt;div class=&quot;image-caption-container&quot;&gt;&lt;h3 class=&quot;image-caption-heading&quot;&gt;Stability of RNase Inhibitor Hu at 37°C over a 6-week period&lt;/h3&gt; &lt;div class=&quot;image-caption-content&quot;&gt;After the given incubation period, 40 U of RNase Inhibitor Hu was incubated with 5 ng of RNase A and 1 μg of RNA for 15 minutes at 37°C.&lt;/div&gt;&lt;/div&gt;" data-src="null" style="color: rgb(0, 77, 159); padding: 0px; border: 0px;"> <span style="padding: 0px; border: 0px; letter-spacing: inherit;">Stability of RNase Inhibitor Hu at 37°C over a 6-week period</span></a>”).</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;">RNase Inhibitor Hu demonstrates stable enzyme activity at 50°C for at least 24 hours. This is applicable for molecular biology applications at elevated temperatures. Enzyme stability at 50°C ensures maximum protection of RNA during reverse transcription or amplification (see figure “<a href="https://www.qiagen.com/binary/resource/BC_1531_ICS_OEM_QPRO_2398_RNase_inhibitor/1_5_WebFullSizeImage.jpg" data-image-pimid="245400" class="lightbox-link" data-sub-html="&lt;div class=&quot;image-caption-container&quot;&gt;&lt;h3 class=&quot;image-caption-heading&quot;&gt;Stability of RNase Inhibitor Hu incubated at 50°C over a 24 hour period&lt;/h3&gt; &lt;div class=&quot;image-caption-content&quot;&gt;After the incubation time 40 U of RNase Inhibitor Hu was incubated with 5 ng of RNase A and 1 μg of RNA for 15 minutes at 37°C&lt;/div&gt;&lt;/div&gt;" data-src="null" style="color: rgb(0, 77, 159); padding: 0px; border: 0px;"> <span style="padding: 0px; border: 0px; letter-spacing: inherit;">Stability of RNase Inhibitor Hu incubated at 50°C over a 24 hour period</span></a>”). Stability in stringent thermal conditions is an important requirement for RNA synthesis and reverse transcription. RNase Inhibitor Human remains fully active for two hours at 60°C (see figure “<a href="https://www.qiagen.com/binary/resource/G_0543_ICS_OEM_QPRO_2398_RNase_inhibitor/1_5_WebFullSizeImage.jpg" data-image-pimid="245393" class="lightbox-link" data-sub-html="&lt;div class=&quot;image-caption-container&quot;&gt;&lt;h3 class=&quot;image-caption-heading&quot;&gt;RNase Inhibitor Hu is thermostable at 60°&lt;/h3&gt; &lt;div class=&quot;image-caption-content&quot;&gt;RNase Inhibitor Hu and a product from an alternative supplier were incubated at 60°C for 2 hours to test stability. At 30-minute time points, 40 U of RNase Inhibitor Hu was incubated with 5 ng of RNase A and 1 g of RNA for 15 minutes at 37°C. Results are shown in 1% agarose gel. Unaffected RNA should present as two clear bands&lt;/div&gt;&lt;/div&gt;" data-src="null" style="color: rgb(0, 77, 159); padding: 0px; border: 0px;"> <span style="padding: 0px; border: 0px; letter-spacing: inherit;">RNase Inhibitor Hu is thermostable at 60°C</span></a>”).</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;">RNase Inhibitor Human retains its activity and RNA protection consistently over a pH range from 5.5 to 9.0 (see figure “<a href="https://www.qiagen.com/binary/resource/G_0546_ICS_OEM_QPRO_2398_RNase_inhibitor/1_5_WebFullSizeImage.jpg" data-image-pimid="245396" class="lightbox-link" data-sub-html="&lt;div class=&quot;image-caption-container&quot;&gt;&lt;h3 class=&quot;image-caption-heading&quot;&gt;RNase Inhibitor Hu works in a wide pH range, from 5.5 to 9.0&lt;/h3&gt; &lt;div class=&quot;image-caption-content&quot;&gt;Results are shown in 1% agarose gel. The RNA was incubated with RNase Inhibitor Hu in buffers with varying pH values. Unaffected RNA should be presented as two clear bands.&lt;/div&gt;&lt;/div&gt;" data-src="null" style="color: rgb(0, 77, 159); padding: 0px; border: 0px;"> <span style="padding: 0px; border: 0px; letter-spacing: inherit;">RNase Inhibitor Hu works in a wide pH range, from 5.5 to 9.0</span></a>”).</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;"><br></p><div style="width: calc(66.6667% - 12px); padding: 0px; border-style: none; border-width: 0px;"></div><div class="rich-data-table-container" style="padding: 0px; border-style: none; border-width: 0px;"><div class="rich-data-table" style="padding: 0px; border-style: none; border-width: 0px;"><table border="1" style="height: 335px; width: 838px; padding: 0px; border: 0px;"><tbody style="padding: 0px; border: 0px;"><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><th style="background: rgb(242, 247, 252); width: 618px; padding: 12px 16px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-weight: 500; font-stretch: normal; font-variant: normal; border-style: none; border-width: 0px;">Assay</th><th style="background: rgb(242, 247, 252); width: 618px; padding: 12px 16px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-weight: 500; font-stretch: normal; font-variant: normal; border-style: none; border-width: 0px;">Specification</th></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 618px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Purity</td><td style="width: 618px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">&gt;90%</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 618px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">RNase contamination</td><td style="width: 618px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">None detected</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 618px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Latent RNase contamination</td><td style="width: 618px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">None detected</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 618px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Exonuclease contamination</td><td style="width: 618px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">None detected</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 618px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Endonuclease contamination</td><td style="width: 618px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">None detected</td></tr></tbody></table></div></div></div></section></wca-product-description></wca-product-details></span></section><p><br style="font-size: 19.0008px;"></p> 491.85 416.82 page_30175 0 0 0