RNase Inhibitor Hu (400000 U)

RNase Inhibitor Hu is a thermally resistant, 50 kDa recombinant human placental protein expressed in Escherichia coli that inhibits RNase activity of common eukaryotic enzymes such as RNase A, RNase B, RNase C.

Native ribonuclease inhibitor is a large (~450 residues, ~50 kDa), acidic (pI ~4.7), leucine-rich repeat protein. Ribonuclease inhibitor shows remarkable affinity for pancreatic-type ribonucleases and acts as a potent noncompetitive inhibitor of these enzymes. The resulting noncovalent complexes are some of the tightest known in biology. Ribonuclease inhibitor does not interfere with the activities of other RNases and modifying enzymes like polymerases and reverse transcriptases.

Ribonuclease inhibitors are essential in molecular biology to minimize the action of ubiquitous ribonucleases that can compromise RNA experiments. The source of RNases within skin, air or labware is primarily microorganisms: bacteria, viruses and fungi.

Benefits of using RNase Inhibitor Human

• Recombinant human enzyme is thermostable and active up to 60°C
• Maximum protection of RNA integrity for intact RNA during handling, storage and assays
• Outperforms alternative supplier products, consistently achieving high RNA integrity
• Suitable for higher temperature applications in RT-PCR, RT-qPCR and RT-LAMP
• Greater resistance to oxidation with more activity at low reducing conditions
• Active over a broad pH range (5.5 to 9.0) and in various buffers
• Improved yields in transcription/translation in in vitro transcription and translation (IVT) systems

RNase Inhibitor Hu is a heat stable recombinant human placental protein expressed in E.coli. RNase Inhibitor Hu retains full stability at 37°C for at least 4 weeks with no loss of activity. 

RNase Inhibitor Hu is supplied with 20 mM Hepes-KOH (pH 7.6), 50 mM KCl, 8 mM reducing agent, 50% (v/v) glycerol. The enzyme is active up to 60°C and at a minimum 0.5–1 mM DTT concentration ranges.

One unit is defined as the amount of enzyme required to inhibit the activity of 5 ng RNase A by 50%.

In applications where RNase inhibitor activity at higher temperatures is less critical, RNase Inhibitor Y9240L is stable up to 50°C and is suitable for use at temperatures ≤50°C. 

RNase Inhibitor Hu outperforms alternative supplier products, consistently achieving high RNA integrity (see figure “ Summary of RNA integrity number (RIN) results for three RNase Inhibitors”).

RNase Inhibitor Hu displays full stability at 37°C for at least 4 weeks. High thermostability guarantees high activity even after exposure to increased temperatures e.g., during worktime on the bench or demanding shipping conditions (see figure “ Stability of RNase Inhibitor Hu at 37°C over a 6-week period”).

RNase Inhibitor Hu demonstrates stable enzyme activity at 50°C for at least 24 hours. This is applicable for molecular biology applications at elevated temperatures. Enzyme stability at 50°C ensures maximum protection of RNA during reverse transcription or amplification (see figure “ Stability of RNase Inhibitor Hu incubated at 50°C over a 24 hour period”). Stability in stringent thermal conditions is an important requirement for RNA synthesis and reverse transcription. RNase Inhibitor Human remains fully active for two hours at 60°C (see figure “ RNase Inhibitor Hu is thermostable at 60°C”).

RNase Inhibitor Human retains its activity and RNA protection consistently over a pH range from 5.5 to 9.0 (see figure “ RNase Inhibitor Hu works in a wide pH range, from 5.5 to 9.0”).