5 U/µL concentration with 10x TaqNova KCl Reaction Buffer, 10x TaqNova (NH4)2SO4 Reaction Buffer, and 50 mM MgCl2 Storage temperature: -20°C
The TaqNova DNA Polymerase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Synthesizes DNA at extremely high temperatures
- Generates high yields with minimal amounts of enzyme and little optimization
- Possesses increased sensitivity and is suitable for a wide range of applications
- Amplifies fragments of up to 5 kb with a half-life of 45 minutes at 95°C
- Leaves ´A´ overhangs
Product Details
TaqNova DNA Polymerase and TaqNova Polymerase UCP are a 94 kDa recombinant, thermostable Taq DNA polymerase isolated from Thermus aquaticus. It is recommended for a wide range of applications that require DNA synthesis at extremely high temperatures. It is a universal, easy-to-use DNA polymerase that works rapidly and effectively in various PCR conditions.
Both enzymes catalyze DNA synthesis in a 5’→3’ direction. It shows no 3’→ 5’ exonuclease activity but has 5’→ 3’ exonuclease activity.
Both enzymes are supplied with 20 mM Tris-HCl (pH 8.0 at 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C for TaqNova DNA Polymerase and at 72°C for TaqNova Polymerase UCP in a 50 μL reaction.
Performance
TaqNova DNA Polymerase UCP
Assay | Specification |
|---|
| Protein Purity | >95% |
| DNase contamination | None detected |
Read more >
126181
RP70
Media/Uploads/RP702A(10).webp
TaqNova DNA Polymerase
TaqNova DNA Polymerase
<p><span style="color: rgb(89, 89, 89); font-size: 16px; font-family: Tahoma; letter-spacing: 0.4px;"><span style="background-color: rgb(255, 255, 255); font-family: "Futura W01", Arial, Helvetica, sans-serif; letter-spacing: 0.25px;">5 U/µL concentration with 10x TaqNova KCl Reaction Buffer, 10x TaqNova (NH</span><span style="background-color: rgb(255, 255, 255); padding: 0px; font-size: 12px; font-family: "Futura W01", Arial, Helvetica, sans-serif; font-stretch: normal; font-variant: normal; border: 0px; letter-spacing: 0.4px;">4</span><span style="background-color: rgb(255, 255, 255); font-family: "Futura W01", Arial, Helvetica, sans-serif; letter-spacing: 0.25px;">)</span><span style="background-color: rgb(255, 255, 255); padding: 0px; font-size: 12px; font-family: "Futura W01", Arial, Helvetica, sans-serif; font-stretch: normal; font-variant: normal; border: 0px; letter-spacing: 0.4px;">2</span><span style="background-color: rgb(255, 255, 255); font-family: "Futura W01", Arial, Helvetica, sans-serif; letter-spacing: 0.25px;">SO</span><span style="background-color: rgb(255, 255, 255); padding: 0px; font-size: 12px; font-family: "Futura W01", Arial, Helvetica, sans-serif; font-stretch: normal; font-variant: normal; border: 0px; letter-spacing: 0.4px;">4</span><span style="background-color: rgb(255, 255, 255); font-family: "Futura W01", Arial, Helvetica, sans-serif; letter-spacing: 0.25px;"> Reaction Buffer, and 50 mM MgCl</span><span style="background-color: rgb(255, 255, 255); padding: 0px; font-size: 12px; font-family: "Futura W01", Arial, Helvetica, sans-serif; font-stretch: normal; font-variant: normal; border: 0px; letter-spacing: 0.4px;">2</span><span style="background-color: rgb(255, 255, 255); font-family: "Futura W01", Arial, Helvetica, sans-serif; letter-spacing: 0.25px;"> Storage temperature: -20°C</span></span></p><p><span style="color: rgb(89, 89, 89); font-size: 16px; font-family: Tahoma; letter-spacing: 0.4px;">The TaqNova DNA Polymerase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.</span></p><h4 _ngcontent-serverapp-c2480474117="" class="section" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 28px; font-family: "Futura W01", Arial, Helvetica, sans-serif; font-stretch: normal; font-variant: normal; border: 0px; letter-spacing: 0px;"><span style="font-size: 16px;"><span style="font-family: Tahoma;">Features</span></span></h4><p><span style="font-size: 16px; font-family: Tahoma;"><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-onedatalayer-source="Product Details" class="ng-star-inserted" style="color: rgb(89, 89, 89); background-color: rgb(255, 255, 255); letter-spacing: 0.25px;"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details></span></p><section _ngcontent-serverapp-c2480474117="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c2480474117="" class="description-box" lg-uid="lg0" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c2480474117="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border-style: none; border-width: 0px; letter-spacing: 0.5px;"><ul style="width: calc(66.6667% - 12px); padding: 0px 0px 0px 18px; border: 0px; list-style-image: initial;"><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Synthesizes DNA at extremely high temperatures</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Generates high yields with minimal amounts of enzyme and little optimization</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Possesses increased sensitivity and is suitable for a wide range of applications</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Amplifies fragments of up to 5 kb with a half-life of 45 minutes at 95°C</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Leaves ´A´ overhangs</li></ul></div></section><section _ngcontent-serverapp-c3508226301="" scrollanchor="product-details" style="color: rgb(89, 89, 89); padding: 0px; font-size: 16px; font-family: "Futura W01", Arial, Helvetica, sans-serif; border: 0px; letter-spacing: 0.25px;"><span style="font-family: Tahoma;"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></span><h4 _ngcontent-serverapp-c2480474117="" class="intro" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 40px; border: 0px;"><span style="font-size: 16px;"><span style="font-family: Tahoma;">Product Details</span></span></h4><span style="font-family: Tahoma;"><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-navigation="Product Details" data-onedatalayer-source="Product Details" class="ng-star-inserted"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"><section _ngcontent-serverapp-c2480474117="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c2480474117="" class="description-box" lg-uid="lg1" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c2480474117="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border-style: none; border-width: 0px; letter-spacing: 0.5px;"><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;">TaqNova DNA Polymerase and TaqNova Polymerase UCP are a 94 kDa recombinant, thermostable Taq DNA polymerase isolated from <em style="padding: 0px; border: 0px;">Thermus aquaticus</em>. It is recommended for a wide range of applications that require DNA synthesis at extremely high temperatures. It is a universal, easy-to-use DNA polymerase that works rapidly and effectively in various PCR conditions.</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;"><br>Both enzymes catalyze DNA synthesis in a 5’→3’ direction. It shows no 3’→ 5’ exonuclease activity but has 5’→ 3’ exonuclease activity.</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;"><br>Both enzymes are supplied with 20 mM Tris-HCl (pH 8.0 at 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;"><br>One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C for TaqNova DNA Polymerase and at 72°C for TaqNova Polymerase UCP in a 50 μL reaction.</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;"><br></p></div></section></wca-product-description></wca-product-details><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-onedatalayer-source="Product Details" class="ng-star-inserted"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details></span><h4 _ngcontent-serverapp-c2480474117="" class="section" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 28px; font-family: "Futura W01", Arial, Helvetica, sans-serif; font-stretch: normal; font-variant: normal; border: 0px; letter-spacing: 0px;"><span style="font-size: 16px;"><span style="font-family: Tahoma;">Performance</span></span></h4><span style="font-family: Tahoma;"><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-onedatalayer-source="Product Details" class="ng-star-inserted"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"><section _ngcontent-serverapp-c2480474117="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c2480474117="" class="description-box" lg-uid="lg2" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c2480474117="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border-style: none; border-width: 0px; letter-spacing: 0.5px;"><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;"><span style="padding: 0px; font-weight: 700; border: 0px;">TaqNova DNA Polymerase UCP</span></p><div style="width: calc(66.6667% - 12px); padding: 0px; border-style: none; border-width: 0px;"></div><div class="rich-data-table-container" style="padding: 0px; border-style: none; border-width: 0px;"><div class="rich-data-table" style="padding: 0px; border-style: none; border-width: 0px;"><table border="1" style="height: 178px; width: 848px; padding: 0px; border: 0px;"><tbody style="padding: 0px; border: 0px;"><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><th style="background: rgb(242, 247, 252); width: 618px; padding: 12px 16px; font-family: "Futura W01", Arial, Helvetica, sans-serif; font-weight: 500; font-stretch: normal; font-variant: normal; border-style: none; border-width: 0px;"><p style="color: inherit; padding: 0px; font-size: inherit; border-style: none; border-width: 0px; letter-spacing: inherit;">Assay</p></th><th style="background: rgb(242, 247, 252); width: 618px; padding: 12px 16px; font-family: "Futura W01", Arial, Helvetica, sans-serif; font-weight: 500; font-stretch: normal; font-variant: normal; border-style: none; border-width: 0px;">Specification</th></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 618px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Protein Purity</td><td style="width: 618px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">>95%</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 618px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">DNase contamination</td><td style="width: 618px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">None detected</td></tr></tbody></table></div></div></div></section></wca-product-description></wca-product-details></span></section><p><br style="font-size: 19.0008px;"></p>
352.58
298.80
page_30166
0
0
0