5 U/µL concentration with 10x TaqNova KCl Reaction Buffer, 10x TaqNova (NH4)2SO4 Reaction Buffer, and 50 mM MgCl2 Storage temperature: -20°C
The TaqNova DNA Polymerase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Synthesizes DNA at extremely high temperatures
- Generates high yields with minimal amounts of enzyme and little optimization
- Possesses increased sensitivity and is suitable for a wide range of applications
- Amplifies fragments of up to 5 kb with a half-life of 45 minutes at 95°C
- Leaves ´A´ overhangs
Product Details
TaqNova DNA Polymerase and TaqNova Polymerase UCP are a 94 kDa recombinant, thermostable Taq DNA polymerase isolated from Thermus aquaticus. It is recommended for a wide range of applications that require DNA synthesis at extremely high temperatures. It is a universal, easy-to-use DNA polymerase that works rapidly and effectively in various PCR conditions.
Both enzymes catalyze DNA synthesis in a 5’→3’ direction. It shows no 3’→ 5’ exonuclease activity but has 5’→ 3’ exonuclease activity.
Both enzymes are supplied with 20 mM Tris-HCl (pH 8.0 at 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C for TaqNova DNA Polymerase and at 72°C for TaqNova Polymerase UCP in a 50 μL reaction.
Performance
TaqNova DNA Polymerase UCP
Assay | Specification |
|---|
| Protein Purity | >95% |
| DNase contamination | None detected |