T4 DNA Ligase (Rapid)

126142 L6030 Media/Uploads/L6030-HC-L(4).webp T4 DNA Ligase (Rapid) T4 DNA Ligase (Rapid) <p><span style="color: rgb(89, 89, 89); font-size: 16px; letter-spacing: 0.4px; font-family: Tahoma;">The T4 DNA Ligase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.</span></p><h4 _ngcontent-serverapp-c2480474117="" class="section" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 28px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px; letter-spacing: 0px;"><span style="font-size: 16px;"><span style="font-family: Tahoma;">Features</span></span></h4><p><span style="font-family: Tahoma; font-size: 16px;"><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-onedatalayer-source="Product Details" class="ng-star-inserted" style="color: rgb(89, 89, 89); background-color: rgb(255, 255, 255); font-size: 16px; letter-spacing: 0.25px;"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details></span></p><section _ngcontent-serverapp-c2480474117="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c2480474117="" class="description-box" lg-uid="lg0" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c2480474117="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border-width: 0px; border-style: none; border-image: initial; letter-spacing: 0.5px;"><ul style="width: calc(66.6667% - 12px); padding: 0px 0px 0px 18px; border: 0px; list-style-type: disc; list-style-image: initial;"><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Versatile enzyme widely used for high-precision NGS library prep and DNA cloning</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Efficiently joins both complementary cohesive (sticky) ends and blunt ends</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Repairs single-stranded nicks in duplex DNA for high-efficiency DNA ligation</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">High-concentration rapid formulation increases efficiency and decreases ligation times</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Quality tested for the absence of DNase contamination</li></ul></div></section><section _ngcontent-serverapp-c3508226301="" scrollanchor="product-details" style="color: rgb(89, 89, 89); padding: 0px; font-size: 16px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; border: 0px; letter-spacing: 0.25px;"><span><span style="font-family: Tahoma;"><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-navigation="Product Details" data-onedatalayer-source="Product Details" class="ng-star-inserted"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details></span></span><h4 _ngcontent-serverapp-c2480474117="" class="intro" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 40px; border: 0px;"><span><span style="font-size: 16px;"><span style="font-family: Tahoma;">Product Details</span></span></span></h4><span><span style="font-family: Tahoma;"><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-navigation="Product Details" data-onedatalayer-source="Product Details" class="ng-star-inserted"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"><section _ngcontent-serverapp-c2480474117="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c2480474117="" class="description-box" lg-uid="lg1" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c2480474117="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border-width: 0px; border-style: none; border-image: initial; letter-spacing: 0.5px;"><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-width: 0px; border-style: none; border-image: initial; letter-spacing: inherit;">T4 DNA Ligase enzyme is a recombinant protein (55,292 Daltons) produced by an E. coli strain carrying the cloned bacteriophage T4 DNA ligase gene.</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-width: 0px; border-style: none; border-image: initial; letter-spacing: inherit;">T4 DNA Ligase is an ATP-dependent enzyme that catalyzes the formation of a phosphodiester bond between the terminal 5ʹ phosphate and a 3' hydroxyl group of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single-stranded nicks in duplex DNA, RNA or DNA:RNA hybrids (1). T4 DNA Ligase is effective for joining blunt or cohesive ends in double-stranded RNA with a complementary "splint" strand (DNA or RNA) holding the RNA ends together. T4 DNA Ligase cannot join single-stranded nucleic acids.</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-width: 0px; border-style: none; border-image: initial; letter-spacing: inherit;"><span style="padding: 0px; font-weight: 700; border: 0px;">Available Configurations:</span></p><div style="width: calc(66.6667% - 12px); padding: 0px; border-width: 0px; border-style: none; border-image: initial;"></div><div class="rich-data-table-container" style="padding: 0px; border-width: 0px; border-style: none; border-image: initial;"><div class="rich-data-table" style="padding: 0px; border-width: 0px; border-style: none; border-image: initial;"><table border="1" style="width: 854px; padding: 0px; border: 0px; height: 457px;"><tbody style="padding: 0px; border: 0px;"><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><th style="background: rgb(242, 247, 252); width: 308.984px; padding: 12px 16px; font-size: 18px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-weight: 500; font-stretch: normal; font-style: normal; font-variant: normal; border-style: none; border-width: 0px;"><span style="padding: 0px; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px;">Feature</span></th><th style="background: rgb(242, 247, 252); width: 308.984px; padding: 12px 16px; font-size: 18px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-weight: 500; font-stretch: normal; font-style: normal; font-variant: normal; border-style: none; border-width: 0px;"><span style="padding: 0px; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px;">T4 DNA Ligase (Standard)</span></th><th style="background: rgb(242, 247, 252); width: 308.984px; padding: 12px 16px; font-size: 18px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-weight: 500; font-stretch: normal; font-style: normal; font-variant: normal; border-style: none; border-width: 0px;"><span style="padding: 0px; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px;">T4 DNA Ligase (Rapid)</span></th><th style="background: rgb(242, 247, 252); width: 309.047px; padding: 12px 16px; font-size: 18px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-weight: 500; font-stretch: normal; font-style: normal; font-variant: normal; border-style: none; border-width: 0px;"><span style="padding: 0px; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px;">WGS Ligase</span></th></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Catalog number</td><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">L6030-LC-L</td><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">L6030-HC-L</td><td style="width: 309.047px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">L6030-W-L</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Best for</td><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Routine cloning and ligation of cohesive or blunt DNA ends</td><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Fast ligation and improved blunt-end efficiency</td><td style="width: 309.047px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Whole genome sequencing (WGS) library construction and adapter ligation</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Ligase concentration</td><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">120,000 U/mL</td><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">600,000 U/mL</td><td style="width: 309.047px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">600,000 U/mL</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Buffers included<br>(PEG concentration)</td><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">10x T4 DNA Ligase Buffer</td><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">10x T4 DNA Ligase Buffer<br>2x Rapid Ligation Buffer (15% PEG 6000)</td><td style="width: 309.047px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">5x Rapid Ligation Buffer (30% PEG 6000)</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Ligation time</td><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">30 minutes at 25°C</td><td style="width: 308.984px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">10 minutes at 25°C</td><td style="width: 309.047px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">15 minutes at 20°C</td></tr></tbody></table></div></div><div style="width: calc(66.6667% - 12px); padding: 0px; border-width: 0px; border-style: none; border-image: initial;"></div><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-width: 0px; border-style: none; border-image: initial; letter-spacing: inherit;"><span style="padding: 0px; font-weight: 700; border: 0px;">Product Composition:</span></p><div style="width: calc(66.6667% - 12px); padding: 0px; border-width: 0px; border-style: none; border-image: initial;"></div><div class="rich-data-table-container" style="padding: 0px; border-width: 0px; border-style: none; border-image: initial;"><div class="rich-data-table" style="padding: 0px; border-width: 0px; border-style: none; border-image: initial;"><table border="1" style="width: 853px; padding: 0px; border: 0px; height: 432px;"><tbody style="padding: 0px; border: 0px;"><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><th style="background: rgb(242, 247, 252); width: 412px; padding: 12px 16px; font-size: 18px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-weight: 500; font-stretch: normal; font-style: normal; font-variant: normal; border-style: none; border-width: 0px;"><span style="padding: 0px; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px;">Component</span></th><th style="background: rgb(242, 247, 252); width: 412px; padding: 12px 16px; font-size: 18px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-weight: 500; font-stretch: normal; font-style: normal; font-variant: normal; border-style: none; border-width: 0px;"><span style="padding: 0px; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px;">Composition</span></th><th style="background: rgb(242, 247, 252); width: 412px; padding: 12px 16px; font-size: 18px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-weight: 500; font-stretch: normal; font-style: normal; font-variant: normal; border-style: none; border-width: 0px;"><span style="padding: 0px; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px;">Storage temperature</span></th></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">T4 DNA Ligase (L6030)</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol; pH 7.5 at 25°C.</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">–25°C to –15°C</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">10x T4 DNA Ligase Buffer (B6030)</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">500 mM Tris-HCI, 100 mM MgCl<sub style="padding: 0px; font-size: 12px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px; letter-spacing: 0.4px;">2</sub>, 50 mM DTT and 10 mM ATP; pH 7.6 at 25°C.</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">–25°C to –15°C</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">2x Rapid Ligation Buffer (B1010)</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">132 mM Tris-HCl, 20 mM MgCl<sub style="padding: 0px; font-size: 12px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px; letter-spacing: 0.4px;">2</sub>, 2 mM DTT, 2 mM ATP and 15% PEG 6000; pH 7.6 at 25°C.</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">–25°C to –15°C</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">5x Rapid Ligation Buffer (B9020)</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">330 mM Tris-HCl, 50mM MgCl<sub style="padding: 0px; font-size: 12px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px; letter-spacing: 0.4px;">2</sub> , 5 mM DTT, 5 mM ATP and 30% PEG 6000; pH 7.6 @ 25°C</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">–25°C to –15°C</td></tr></tbody></table></div></div></div></section></wca-product-description></wca-product-details></span></span></section><p></p> 4662.85 3951.57 page_30127 0 0 0