240,000 U of T4 DNA Ligase (600,000 U/mL) 2x Rapid Ligation Buffer and 10x T4 DNA Ligase Buffer
The T4 DNA Ligase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Versatile enzyme widely used for high-precision NGS library prep and DNA cloning
- Efficiently joins both complementary cohesive (sticky) ends and blunt ends
- Repairs single-stranded nicks in duplex DNA for high-efficiency DNA ligation
- High-concentration rapid formulation increases efficiency and decreases ligation times
- Quality tested for the absence of DNase contamination
T4 DNA Ligase enzyme is a recombinant protein (55,292 Daltons) produced by an E. coli strain carrying the cloned bacteriophage T4 DNA ligase gene.
T4 DNA Ligase is an ATP-dependent enzyme that catalyzes the formation of a phosphodiester bond between the terminal 5ʹ phosphate and a 3' hydroxyl group of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single-stranded nicks in duplex DNA, RNA or DNA:RNA hybrids (1). T4 DNA Ligase is effective for joining blunt or cohesive ends in double-stranded RNA with a complementary "splint" strand (DNA or RNA) holding the RNA ends together. T4 DNA Ligase cannot join single-stranded nucleic acids.
Available Configurations:
| Feature | T4 DNA Ligase (Standard) | T4 DNA Ligase (Rapid) | WGS Ligase |
|---|
| Catalog number | L6030-LC-L | L6030-HC-L | L6030-W-L |
| Best for | Routine cloning and ligation of cohesive or blunt DNA ends | Fast ligation and improved blunt-end efficiency | Whole genome sequencing (WGS) library construction and adapter ligation |
| Ligase concentration | 120,000 U/mL | 600,000 U/mL | 600,000 U/mL |
Buffers included
(PEG concentration) | 10x T4 DNA Ligase Buffer | 10x T4 DNA Ligase Buffer
2x Rapid Ligation Buffer (15% PEG 6000) | 5x Rapid Ligation Buffer (30% PEG 6000) |
| Ligation time | 30 minutes at 25°C | 10 minutes at 25°C | 15 minutes at 20°C |
Product Composition:
| Component | Composition | Storage temperature |
|---|
| T4 DNA Ligase (L6030) | Supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol; pH 7.5 at 25°C. | –25°C to –15°C |
| 10x T4 DNA Ligase Buffer (B6030) | 500 mM Tris-HCI, 100 mM MgCl2, 50 mM DTT and 10 mM ATP; pH 7.6 at 25°C. | –25°C to –15°C |
| 2x Rapid Ligation Buffer (B1010) | 132 mM Tris-HCl, 20 mM MgCl2, 2 mM DTT, 2 mM ATP and 15% PEG 6000; pH 7.6 at 25°C. | –25°C to –15°C |
| 5x Rapid Ligation Buffer (B9020) | 330 mM Tris-HCl, 50mM MgCl2 , 5 mM DTT, 5 mM ATP and 30% PEG 6000; pH 7.6 @ 25°C | –25°C to –15°C |