Saltonas

126128 EN32 Media/Uploads/EN32-050(1).webp Saltonas Saltonas <p><span style="color: rgb(89, 89, 89); font-size: 16px; letter-spacing: 0.4px; font-family: Tahoma;">The Saltonase (MBG and GMP-grade) is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.</span></p><h4 _ngcontent-serverapp-c2480474117="" class="section" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 28px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px; letter-spacing: 0px;"><span style="font-size: 16px;"><span style="font-family: Tahoma;">Features</span></span></h4><p><span style="font-family: Tahoma; font-size: 16px;"><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-onedatalayer-source="Product Details" class="ng-star-inserted" style="color: rgb(89, 89, 89); background-color: rgb(255, 255, 255); font-size: 16px; letter-spacing: 0.25px;"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details></span></p><section _ngcontent-serverapp-c2480474117="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c2480474117="" class="description-box" lg-uid="lg0" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c2480474117="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border-width: 0px; border-style: none; border-image: initial; letter-spacing: 0.5px;"><ul style="width: calc(66.6667% - 12px); padding: 0px 0px 0px 18px; border: 0px; list-style-type: disc; list-style-image: initial;"><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Molecular Biology Grade (MBG) and Good Manufacturing Practice (GMP)-grade</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Highly active in a broad range of temperatures (&gt;20% at 15–55°C)</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Maximum nucleolytic activity at high salt concentrations (optimal concentration for NaCl or KCl is 450–600 mM) and other buffer additives</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Highly active in the typical buffers and grow media</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Requires ≥1 mM Mg<sup style="padding: 0px; font-size: 12px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px; letter-spacing: 0.4px;">2+</sup> to activate and shows a broad spectrum of pH activity (optimum at pH 8.5, working pH range &gt;with enzyme activity ≥20% 6.8–9.3)</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Used together with <a href="https://www.qiagen.com/us/products/discovery-and-translational-research/enzymes-for-molecular-biology/elisa?catno=EN32-001" data-image-pimid="206954" style="color: rgb(0, 77, 159); padding: 0px; border: 0px;">Saltonase® ELISA Kit</a>, they streamline both the enzymatic digestion of nucleic acids and the detection of residual nuclease</li></ul></div></section><section _ngcontent-serverapp-c3508226301="" scrollanchor="product-details" style="color: rgb(89, 89, 89); padding: 0px; font-size: 16px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; border: 0px; letter-spacing: 0.25px;"><span><span style="font-family: Tahoma;"><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-navigation="Product Details" data-onedatalayer-source="Product Details" class="ng-star-inserted"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details></span></span><h4 _ngcontent-serverapp-c2480474117="" class="intro" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 40px; border: 0px;"><span><span style="font-size: 16px;"><span style="font-family: Tahoma;">Product Details</span></span></span></h4><span><span style="font-family: Tahoma;"><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-navigation="Product Details" data-onedatalayer-source="Product Details" class="ng-star-inserted"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"><section _ngcontent-serverapp-c2480474117="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c2480474117="" class="description-box" lg-uid="lg1" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c2480474117="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border-width: 0px; border-style: none; border-image: initial; letter-spacing: 0.5px;"><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-width: 0px; border-style: none; border-image: initial; letter-spacing: inherit;">Saltonase is a recombinant endonuclease derived from psychrophilic bacteria and produced in <em style="padding: 0px; border: 0px;">E. coli</em>. It is highly active across a wide range of salt concentrations and pH levels, making it effective for digesting both DNA and RNA substrates under various buffer conditions and temperatures. Saltonase demonstrates robust activity in challenging environments, including those with high salt content and a broad pH range. These characteristics make Saltonase an invaluable tool for eliminating undesired nucleic acid contamination during protein purification processes in both laboratory and biomanufacturing workflows.&nbsp;</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-width: 0px; border-style: none; border-image: initial; letter-spacing: inherit;">It is supplied with 20 mM Tris (pH 7.1 w 25°C), 620 mM NaCl, 26 mM MgCl<sub style="padding: 0px; font-size: 12px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px; letter-spacing: 0.4px;">2</sub> and 50% (v/v) glycerol.&nbsp;</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-width: 0px; border-style: none; border-image: initial; letter-spacing: inherit;">One unit is defined as an increase in absorbance at 260 nm of 1.0 in 30 minutes at 37°C in 50 mM Tris-HCl buffer, pH 8.5 (25°C) supplemented with 500 mM NaCl, 5 mM MgCl<sub style="padding: 0px; font-size: 12px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px; letter-spacing: 0.4px;">2</sub>, 0.1 mg/mL BSA and 0.5 mg/mL herring sperm DNA as a substrate. </p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border-width: 0px; border-style: none; border-image: initial; letter-spacing: inherit;">&nbsp;</p></div></section></wca-product-description></wca-product-details><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-onedatalayer-source="Product Details" class="ng-star-inserted"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details></span></span><h4 _ngcontent-serverapp-c2480474117="" class="section" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 28px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; font-style: normal; font-variant: normal; border: 0px; letter-spacing: 0px;"><span><span style="font-size: 16px;"><span style="font-family: Tahoma;">Performance</span></span></span></h4><span><span style="font-family: Tahoma;"><wca-product-details _ngcontent-serverapp-c3508226301="" _nghost-serverapp-c1495514609="" data-onedatalayer-source="Product Details" class="ng-star-inserted"><wca-product-description _ngcontent-serverapp-c1495514609="" _nghost-serverapp-c2480474117="" data-onedatalayer-source="Product Description" class="ng-star-inserted"><section _ngcontent-serverapp-c2480474117="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c2480474117="" class="description-box" lg-uid="lg2" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c2480474117="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border-width: 0px; border-style: none; border-image: initial; letter-spacing: 0.5px;"><div style="width: calc(66.6667% - 12px); padding: 0px; border-width: 0px; border-style: none; border-image: initial;"></div><div class="rich-data-table-container" style="padding: 0px; border-width: 0px; border-style: none; border-image: initial;"><div class="rich-data-table" style="padding: 0px; border-width: 0px; border-style: none; border-image: initial;"><table border="1" style="width: 851px; padding: 0px; border: 0px; height: 389px;"><tbody style="padding: 0px; border: 0px;"><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Saltonase</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Molecular Biology Grade</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">GMP-grade*</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Activity</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">≥250 U/µL</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">≥250 U/µL</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Purity</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">≥95 %</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">≥99 %</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Protein purity</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">SDS-PAGE</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">HPLC and SDS-PAGE</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Protease activity</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">None detected</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">None detected</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Endotoxins</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Not tested</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">≤0.25 EU/1000 U</td></tr><tr style="padding: 0px; border-width: 1px 0px; border-style: solid none; border-color: rgb(188, 188, 187) currentcolor;"><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Bioburden</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">Not tested</td><td style="width: 412px; padding: 12px 16px; border-style: none; border-width: 0px; text-align: left;">≤10 CFU/100,000 U</td></tr></tbody></table></div></div></div></section></wca-product-description></wca-product-details></span></span></section><p></p> 700.91 593.99 page_30117 0 0 0