The Saltonase (MBG and GMP-grade) is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Molecular Biology Grade (MBG) and Good Manufacturing Practice (GMP)-grade
- Highly active in a broad range of temperatures (>20% at 15–55°C)
- Maximum nucleolytic activity at high salt concentrations (optimal concentration for NaCl or KCl is 450–600 mM) and other buffer additives
- Highly active in the typical buffers and grow media
- Requires ≥1 mM Mg2+ to activate and shows a broad spectrum of pH activity (optimum at pH 8.5, working pH range >with enzyme activity ≥20% 6.8–9.3)
- Used together with Saltonase® ELISA Kit, they streamline both the enzymatic digestion of nucleic acids and the detection of residual nuclease
Product Details
Saltonase is a recombinant endonuclease derived from psychrophilic bacteria and produced in E. coli. It is highly active across a wide range of salt concentrations and pH levels, making it effective for digesting both DNA and RNA substrates under various buffer conditions and temperatures. Saltonase demonstrates robust activity in challenging environments, including those with high salt content and a broad pH range. These characteristics make Saltonase an invaluable tool for eliminating undesired nucleic acid contamination during protein purification processes in both laboratory and biomanufacturing workflows.
It is supplied with 20 mM Tris (pH 7.1 w 25°C), 620 mM NaCl, 26 mM MgCl2 and 50% (v/v) glycerol.
One unit is defined as an increase in absorbance at 260 nm of 1.0 in 30 minutes at 37°C in 50 mM Tris-HCl buffer, pH 8.5 (25°C) supplemented with 500 mM NaCl, 5 mM MgCl2, 0.1 mg/mL BSA and 0.5 mg/mL herring sperm DNA as a substrate.
Performance
| Saltonase | Molecular Biology Grade | GMP-grade* |
| Activity | ≥250 U/µL | ≥250 U/µL |
| Purity | ≥95 % | ≥99 % |
| Protein purity | SDS-PAGE | HPLC and SDS-PAGE |
| Protease activity | None detected | None detected |
| Endotoxins | Not tested | ≤0.25 EU/1000 U |
| Bioburden | Not tested | ≤10 CFU/100,000 U |