The NEXTFLEX® NGS Cleanup Beads are Solid-phase reversible immobilization (SPRI) beads that efficiently purify nucleic acids using reversible magnetic bead binding. They are ideal for PCR and NGS cleanup, removing unwanted components like soluble contaminants, nucleotides, primers, salts, and adapters. They selectively bind DNA by size with high recovery and consistent results. These SPRI beads can be used manually or in any automated liquid handler, depending on sample throughput.
The NEXTFLEX NGS Cleanup Beads are a cost-effective solution for these applications:
- NGS Library Prep protocols
- Any protocols requiring the removal of unwanted components such as primers, salts or size selection of DNA
- Drop-in replacement for most SPRI beads
Important attributes:
- Input: Amplicons, fragmented gDNA
- Applications: PCR Purification, DNA Cleanup, NGS Cleanup
- Recovery: 70-90% for dsDNA >100 bp
- Size Selection: Please check what Bead-to-sample Ratio is and why it matters
- Storage: 4ºC
Features:
- Drop in AMPure XP replacement: NEXTFLEX beads use the same paramagnetic SPRI chemistry
- Tunable size selection : adjust the bead ratio for left , right or double side clean ups, eliminating gels or chips. Benchmarked performance matches AMPure XP and SPRIselect.
- Platform compatibility: Most NGS library prep kits including NEXTFLEX®
Performance
Figure 1 shows an example of size selection performed with NEXTFLEX® NGS Cleanup and a popular competitor using different bead-to-sample ratios. Results show a predictable and consistent size selection, comparable to competition.
Figure 1. 625 ng of 50 bp DNA ladder (Promega) were purified with equal amount of AMPure® XP (Beckman) and NEXTFLEX® NGS Cleanup and run on 1% agarose gel for comparison.
Figure 2. shows final yields obtained when using same bead-to-sample ratio of either the NEXTFLEX® NGS Cleanup or a popular competitor to cleanup the libraries generated with our NEXTFLEX 16S V4 2.0 kit. Yields of both solutions are not significantly different
Specifications
| Automation Compatible | Yes |
|---|
| Product Group | |
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| Shipping Conditions | Shipped Ambient |
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| Unit Size | 1 mL |
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Read more >
125862NOVA-BEADSMedia/Uploads/NOVA-BEADS-1ML(4).webpNEXTFLEX NGS CLEANUP BEADS 1-500MLNEXTFLEX NGS CLEANUP BEADS<section id="field_rich_text_label_1" style="color: rgb(0, 0, 0); background: rgb(255, 255, 255); padding: 0px 0px 40px; font-size: 16px; font-family: "Hanken Grotesk", sans-serif; font-stretch: inherit; border-width: 0px 0px 1px; border-top-style: initial; border-top-color: initial; border-right-style: initial; border-right-color: initial; border-bottom-style: solid; border-bottom-color: rgb(168, 168, 168); border-left-style: initial; border-left-color: initial;"><div class="panel-overview" style="background: transparent; padding: 0px; border: 0px;"><div class="all-description specifications" style="background: transparent; padding: 0px; border: 0px;"><div style="background: transparent; padding: 0px; border: 0px;"><p style="color: rgb(0, 0, 0); background: rgb(255, 255, 255); padding: 0px; font-size: 18px; font-family: "Hanken Grotesk", sans-serif; border: 0px;"><span style="color: rgb(0, 0, 0); font-size: 16px; font-family: "Hanken Grotesk", sans-serif;">The NEXTFLEX</span><sup style="color: rgb(0, 0, 0); padding: 0px; font-family: "Hanken Grotesk", sans-serif; border: 0px;">®</sup><span style="color: rgb(0, 0, 0); font-size: 16px; font-family: "Hanken Grotesk", sans-serif;"> NGS Cleanup Beads are Solid-phase reversible immobilization (SPRI) beads that efficiently purify nucleic acids using reversible magnetic bead binding. They are ideal for PCR and NGS cleanup, removing unwanted components like soluble contaminants, nucleotides, primers, salts, and adapters. They selectively bind DNA by size with high recovery and consistent results. These SPRI beads can be used manually or in any automated liquid handler, depending on sample throughput.</span></p><p style="color: rgb(0, 0, 0); background: rgb(255, 255, 255); padding: 0px; font-size: 18px; font-family: "Hanken Grotesk", sans-serif; border: 0px;">The NEXTFLEX NGS Cleanup Beads are a cost-effective solution for these applications:</p><ul style="color: rgb(0, 0, 0); background: rgb(255, 255, 255); padding: 0px; font-size: 16px; font-family: "Hanken Grotesk", sans-serif; border: 0px; list-style-type: none; list-style-image: initial;"><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;">NGS Library Prep protocols</li><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;">Any protocols requiring the removal of unwanted components such as primers, salts or size selection of DNA</li><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;">Drop-in replacement for most SPRI beads</li></ul><h3 class="rvty-heading-h5" style="color: rgb(0, 0, 0); background: rgb(255, 255, 255); padding: 0px; font-size: 24px; font-family: "Hanken Grotesk", sans-serif; font-weight: 500; border: 0px;">Important attributes:<br></h3><ul style="color: rgb(0, 0, 0); background: rgb(255, 255, 255); padding: 0px; font-size: 16px; font-family: "Hanken Grotesk", sans-serif; border: 0px; list-style-type: none; list-style-image: initial;"><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;"><strong style="background: transparent; padding: 0px; border: 0px;">Input: </strong>Amplicons, fragmented gDNA</li><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;"><strong style="background: transparent; padding: 0px; border: 0px;">Applications:</strong> PCR Purification, DNA Cleanup, NGS Cleanup</li><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;"><strong style="background: transparent; padding: 0px; border: 0px;">Recovery: </strong>70-90% for dsDNA >100 bp</li><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;"><strong style="background: transparent; padding: 0px; border: 0px;">Size Selection: </strong>Please check what <a href="https://www.revvity.com/blog/spri-technology-principles-bead-composition-and-bead-sample-ratio-impact" data-once="rvty-link-element" data-cta-link-name="Bead-to-sample Ratio" style="color: rgb(127, 3, 176); padding: 0px; border: 0px;">Bead-to-sample Ratio</a> is and why it matters</li><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;"><strong style="background: transparent; padding: 0px; border: 0px;">Storage:</strong> 4ºC</li></ul><h3 class="rvty-heading-h5" style="background: transparent; padding: 0px; font-size: 24px; font-family: "Hanken Grotesk", sans-serif; font-weight: 500; border: 0px;">Features:</h3><ul style="background: transparent; padding: 0px; border: 0px; list-style-type: none; list-style-image: initial;"><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;"><strong style="background: transparent; padding: 0px; border: 0px;">Drop in AMPure XP replacement:</strong> NEXTFLEX beads use the same paramagnetic SPRI chemistry</li><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;"><strong style="background: transparent; padding: 0px; border: 0px;">Tunable size selection : </strong>adjust the bead ratio for left , right or double side clean ups, eliminating gels or chips. Benchmarked performance matches AMPure XP and SPRIselect.</li><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;"><strong style="background: transparent; padding: 0px; border: 0px;">Platform compatibility: </strong>Most NGS library prep kits including NEXTFLEX®<br> </li></ul><div class="rvty-remote-image-outer rvty-remote-image-default " data-once="rvty-add-target-attribute remoteImageAddLink" style="background: transparent; padding: 0px; border: 0px;"><article tabindex="0" class="rvty-remote-img-exp-article rvty-remote-img-exp-border" data-once="remoteImageStyles" style="background: transparent; padding: 0px; font-size: inherit; font-family: inherit; font-weight: inherit; font-stretch: inherit; font-style: inherit; font-variant: inherit; border: 1px solid rgb(227, 227, 227);"><picture style="height: 283px;"><img src="https://resources.revvity.com/images/nextflex-ngs-cleanup-beads-workflow.png?format=auto" height="auto" width="100%" alt="nextflex-ngs-cleanup-beads-workflow" class="rvty-remote-img-exp-img" data-original="https://resources.revvity.com/images/nextflex-ngs-cleanup-beads-workflow.png?format=auto" loading="lazy" style="background: transparent; height: auto; width: 902px; padding: 0px;"></picture><button tabindex="0" class="rvty-remote-img-exp-btn rvty-focus-visible" aria-label="Expand image" style="background: rgba(255, 255, 255, 0.5); height: 24px; width: 24px; padding: 0px; font-size: 16px; border-width: initial; border-style: none; border-color: initial; text-align: left;"><svg width="24" height="24" viewBox="0 0 24 24" fill="none" data-listid=""><path d="M15 3H21V9" stroke="black" stroke-width="2" stroke-linecap="round" stroke-linejoin="round" data-listid=""></path><path d="M9 21H3V15" stroke="black" stroke-width="2" stroke-linecap="round" stroke-linejoin="round" data-listid=""></path><path d="M21 3L14 10" stroke="black" stroke-width="2" stroke-linecap="round" stroke-linejoin="round" data-listid=""></path><path d="M3 21L10 14" stroke="black" stroke-width="2" stroke-linecap="round" stroke-linejoin="round" data-listid=""></path></svg></button></article></div><p style="background: transparent; padding: 0px; font-size: 18px; border: 0px;"> </p><h3 class="rvty-heading-h5" style="background: transparent; padding: 0px; font-size: 24px; font-family: "Hanken Grotesk", sans-serif; font-weight: 500; border: 0px;">Performance</h3><p style="background: transparent; padding: 0px; font-size: 18px; border: 0px;">Figure 1 shows an example of size selection performed with NEXTFLEX® NGS Cleanup and a popular competitor using different bead-to-sample ratios. Results show a predictable and consistent size selection, comparable to competition.</p><div class="rvty-remote-image-outer rvty-remote-image-default " data-once="rvty-add-target-attribute remoteImageAddLink" style="background: transparent; padding: 0px; border: 0px;"><article tabindex="0" class="rvty-remote-img-exp-article rvty-remote-img-exp-border" data-once="remoteImageStyles" style="background: transparent; padding: 0px; font-size: inherit; font-family: inherit; font-weight: inherit; font-stretch: inherit; font-style: inherit; font-variant: inherit; border: 1px solid rgb(227, 227, 227);"><picture style="height: 527px;"><img src="https://resources.revvity.com/images/nextflex-ampure-dna-ladder-comparison.png?format=auto" height="auto" width="100%" alt="nextflex-ampure-dna-ladder-comparison" class="rvty-remote-img-exp-img" data-original="https://resources.revvity.com/images/nextflex-ampure-dna-ladder-comparison.png?format=auto" loading="lazy" style="background: transparent; height: auto; width: 902px; padding: 0px;"></picture><button tabindex="0" class="rvty-remote-img-exp-btn rvty-focus-visible" aria-label="Expand image" style="background: rgba(255, 255, 255, 0.5); height: 24px; width: 24px; padding: 0px; font-size: 16px; border-width: initial; border-style: none; border-color: initial; text-align: left;"><svg width="24" height="24" viewBox="0 0 24 24" fill="none" data-listid=""><path d="M15 3H21V9" stroke="black" stroke-width="2" stroke-linecap="round" stroke-linejoin="round" data-listid=""></path><path d="M9 21H3V15" stroke="black" stroke-width="2" stroke-linecap="round" stroke-linejoin="round" data-listid=""></path><path d="M21 3L14 10" stroke="black" stroke-width="2" stroke-linecap="round" stroke-linejoin="round" data-listid=""></path><path d="M3 21L10 14" stroke="black" stroke-width="2" stroke-linecap="round" stroke-linejoin="round" data-listid=""></path></svg></button></article></div><p style="background: transparent; padding: 0px; font-size: 18px; border: 0px;"><br><em style="background: transparent; padding: 0px; border: 0px;">Figure 1. 625 ng of 50 bp DNA ladder (Promega) were purified with equal amount of AMPure® XP (Beckman) and NEXTFLEX® NGS Cleanup and run on 1% agarose gel for comparison.</em></p><div class="rvty-remote-image-outer rvty-remote-image-default " data-once="rvty-add-target-attribute remoteImageAddLink" style="background: transparent; padding: 0px; border: 0px;"><article tabindex="0" class="rvty-remote-img-exp-article rvty-remote-img-exp-border" data-once="remoteImageStyles" style="background: transparent; padding: 0px; font-size: inherit; font-family: inherit; font-weight: inherit; font-stretch: inherit; font-style: inherit; font-variant: inherit; border: 1px solid rgb(227, 227, 227);"><picture style="height: 653px;"><img src="https://resources.revvity.com/images/Bead-Chart.png?format=auto" height="auto" width="100%" alt="Bead-Chart" class="rvty-remote-img-exp-img" data-original="https://resources.revvity.com/images/Bead-Chart.png?format=auto" loading="lazy" style="background: transparent; height: auto; width: 902px; padding: 0px;"></picture><button tabindex="0" class="rvty-remote-img-exp-btn rvty-focus-visible" aria-label="Expand image" style="background: rgba(255, 255, 255, 0.5); height: 24px; width: 24px; padding: 0px; font-size: 16px; border-width: initial; border-style: none; border-color: initial; text-align: left;"><svg width="24" height="24" viewBox="0 0 24 24" fill="none" data-listid=""><path d="M15 3H21V9" stroke="black" stroke-width="2" stroke-linecap="round" stroke-linejoin="round" data-listid=""></path><path d="M9 21H3V15" stroke="black" stroke-width="2" stroke-linecap="round" stroke-linejoin="round" data-listid=""></path><path d="M21 3L14 10" stroke="black" stroke-width="2" stroke-linecap="round" stroke-linejoin="round" data-listid=""></path><path d="M3 21L10 14" stroke="black" stroke-width="2" stroke-linecap="round" stroke-linejoin="round" data-listid=""></path></svg></button></article></div><p style="background: transparent; padding: 0px; font-size: 18px; border: 0px;"><br><em style="background: transparent; padding: 0px; border: 0px;">Figure 2. shows final yields obtained when using same bead-to-sample ratio of either the NEXTFLEX® NGS Cleanup or a popular competitor to cleanup the libraries generated with our NEXTFLEX 16S V4 2.0 kit. Yields of both solutions are not significantly different</em></p></div></div></div></section><section id="specifications" style="color: rgb(0, 0, 0); background: rgb(255, 255, 255); padding: 0px; font-size: 16px; font-family: "Hanken Grotesk", sans-serif; font-stretch: inherit; border: 0px;"><h3 class="rvty-heading-h4 overview-title" style="background: transparent; padding: 0px; font-size: 32px; font-family: "Hanken Grotesk", sans-serif; font-weight: 500; border: 0px;">Specifications</h3><div class="panel-overview" style="background: transparent; padding: 0px; border: 0px;"><div class="specifications" style="background: transparent; padding: 0px; border: 0px;"></div><div class="specifications" style="background: transparent; padding: 0px; border: 0px;"><div class="field-group-table group_other_specifications" style="background: transparent; padding: 0px; border: 0px;"><div class="table-header" style="background: transparent; padding: 0px; border: 0px;"><label class="table-label" style="background: transparent; padding: 0px; font-size: 24px; border: 0px;">Other specifications</label></div><table class="table node-product-variation-view" style="width: 904px; padding: 0px; border-top: 1px solid rgb(227, 227, 227); border-left: 1px solid rgb(227, 227, 227); border-right-color: rgb(227, 227, 227); border-bottom-color: rgb(227, 227, 227); border-right-style: solid; border-bottom-style: solid;"><tbody style="background: transparent; padding: 0px; border: 0px;"><tr class="field-automation-compatible type-entity-reference table-row" style="background: transparent; width: 902px; padding: 0px; border: 0px none;"><th style="background: rgb(251, 251, 251); width: 225.5px; padding: 12px; font-weight: 400; border-width: 0px 1px 0px 0px; border-top-style: initial; border-top-color: initial; border-right-style: solid; border-right-color: rgb(227, 227, 227); border-bottom-style: initial; border-bottom-color: initial; border-left-style: initial; border-left-color: initial;">Automation Compatible</th><td style="background: transparent; width: 676.5px; padding: 12px; border: 0px; text-align: left;"><div style="background: transparent; padding: 0px; border: 0px;">Yes</div></td></tr><tr class="field-product-group type-entity-reference table-row" style="background: transparent; width: 902px; padding: 0px; border-width: 1px 0px 0px; border-top-style: solid; border-top-color: rgb(227, 227, 227); border-right-style: initial; border-right-color: initial; border-bottom-style: initial; border-bottom-color: initial; border-left-style: initial; border-left-color: initial;"><th style="background: rgb(251, 251, 251); width: 225.5px; padding: 12px; font-weight: 400; border-width: 0px 1px 0px 0px; border-top-style: initial; border-top-color: initial; border-right-style: solid; border-right-color: rgb(227, 227, 227); border-bottom-style: initial; border-bottom-color: initial; border-left-style: initial; border-left-color: initial;">Product Group</th><td style="background: transparent; width: 676.5px; padding: 12px; border: 0px; text-align: left;"><div style="background: transparent; padding: 0px; border: 0px;"><div style="background: transparent; padding: 0px; border: 0px;">Accessories</div></div></td></tr><tr class="field-shipping-conditions-descri type-entity-reference table-row" style="background: transparent; width: 902px; padding: 0px; border-width: 1px 0px 0px; border-top-style: solid; border-top-color: rgb(227, 227, 227); border-right-style: initial; border-right-color: initial; border-bottom-style: initial; border-bottom-color: initial; border-left-style: initial; border-left-color: initial;"><th style="background: rgb(251, 251, 251); width: 225.5px; padding: 12px; font-weight: 400; border-width: 0px 1px 0px 0px; border-top-style: initial; border-top-color: initial; border-right-style: solid; border-right-color: rgb(227, 227, 227); border-bottom-style: initial; border-bottom-color: initial; border-left-style: initial; border-left-color: initial;">Shipping Conditions</th><td style="background: transparent; width: 676.5px; padding: 12px; border: 0px; text-align: left;"><div style="background: transparent; padding: 0px; border: 0px;">Shipped Ambient</div></td></tr><tr class="field-unit-size type-string table-row" style="background: transparent; width: 902px; padding: 0px; border-width: 1px 0px 0px; border-top-style: solid; border-top-color: rgb(227, 227, 227); border-right-style: initial; border-right-color: initial; border-bottom-style: initial; border-bottom-color: initial; border-left-style: initial; border-left-color: initial;"><th style="background: rgb(251, 251, 251); width: 225.5px; padding: 12px; font-weight: 400; border-width: 0px 1px 0px 0px; border-top-style: initial; border-top-color: initial; border-right-style: solid; border-right-color: rgb(227, 227, 227); border-bottom-style: initial; border-bottom-color: initial; border-left-style: initial; border-left-color: initial;">Unit Size</th><td style="background: transparent; width: 676.5px; padding: 12px; border: 0px; text-align: left;"><div style="background: transparent; padding: 0px; border: 0px;">1 mL</div></td></tr></tbody></table></div></div></div></section>38947.7933006.60page_30038000870BEADS NOVA-5002130/09/202510000