The NEXTFLEX® NGS Cleanup Beads are Solid-phase reversible immobilization (SPRI) beads that efficiently purify nucleic acids using reversible magnetic bead binding. They are ideal for PCR and NGS cleanup, removing unwanted components like soluble contaminants, nucleotides, primers, salts, and adapters. They selectively bind DNA by size with high recovery and consistent results. These SPRI beads can be used manually or in any automated liquid handler, depending on sample throughput.
The NEXTFLEX NGS Cleanup Beads are a cost-effective solution for these applications:
- NGS Library Prep protocols
- Any protocols requiring the removal of unwanted components such as primers, salts or size selection of DNA
- Drop-in replacement for most SPRI beads
Important attributes:
- Input: Amplicons, fragmented gDNA
- Applications: PCR Purification, DNA Cleanup, NGS Cleanup
- Recovery: 70-90% for dsDNA >100 bp
- Size Selection: Please check what Bead-to-sample Ratio is and why it matters
- Storage: 4ºC
Features:
- Drop in AMPure XP replacement: NEXTFLEX beads use the same paramagnetic SPRI chemistry
- Tunable size selection : adjust the bead ratio for left , right or double side clean ups, eliminating gels or chips. Benchmarked performance matches AMPure XP and SPRIselect.
- Platform compatibility: Most NGS library prep kits including NEXTFLEX®
Performance
Figure 1 shows an example of size selection performed with NEXTFLEX® NGS Cleanup and a popular competitor using different bead-to-sample ratios. Results show a predictable and consistent size selection, comparable to competition.
Figure 1. 625 ng of 50 bp DNA ladder (Promega) were purified with equal amount of AMPure® XP (Beckman) and NEXTFLEX® NGS Cleanup and run on 1% agarose gel for comparison.
Figure 2. shows final yields obtained when using same bead-to-sample ratio of either the NEXTFLEX® NGS Cleanup or a popular competitor to cleanup the libraries generated with our NEXTFLEX 16S V4 2.0 kit. Yields of both solutions are not significantly different