A product that can separate/purify LPS existing on the outer wall of Gram-negative bacteria • Isolation/purification of LPS in Gram-negative bacteria • Phenol-water method of LPS extraction • Effective removal of contaminants such as proteins and nucleic acids • The most efficient and simple LPS extraction product • The entire process of extracting LPS is completed in up to 60 minutes LPS Extraction Kit is a product that can separate/purify LPS existing on the outer cell wall of Gram-negative bacteria. In general, Gram-negative bacteria have a separate structure called lipopolysaccharide (LPS) in addition to phospholipid and protein in the external layer of the cell envelope, and this has different characteristics for each bacteria. Such LPS is known to play an important role in bacterial growth and survival, and especially in the interaction between host and parasite. In addition, LPS is understood to be closely related to the immune response, such as causing peripheral vascular collapse by secreting endotoxin when septic shock is applied, and is also important for toxic pathophysiological responses. The extraction pattern of LPS is different for each bacteria, and through this, the bacteria are systematically separated. As mentioned earlier, bacterial endotoxins are generally present on the outer walls of Gram-negative bacteria and are not expressed while the bacteria are alive, but are released to the outside by breaking the walls of the cells when the bacteria proliferate or die. Natural entotoxin is composed of LPS, Proteins, and Phospholipids. It is very stable in nature, resistant to heat, negatively charged, and has a large molecular weight (more than 1,000,000 daltons). In general, proteins are extracted with Trichloroacetic acid, Butanol or EDTA to remove lipid associated proteins (LAP), and phospholipids are extracted using phenol. Typically, if you try to extract LPS, you can get around 1-4% LPS per bacterial dry weight.Since the LPS Extraction Kit is based on the Phenol water method, it denatures the protein in entotoxin and removes it by denaturing it in the form of a precipitate between the aqueous layer and the phenol layer.Since the lipid is separated by the phenol/chloroform fraction, only high LPS can be extracted quickly and easily. Applications 01 LPS composition and structure study 02 Phylogenetic study of bacteria 03 Antibiotic target research 04 LPS inhibitory drug design study 05 Carbohydrate antigen immune response study