MEM (Minimum Essential Medium) with Earle's Salts was originally developed by Harry Eagle and from there modified in several ways (e.g. alpha modification) to suit many cell culture applications. It is used for cultivation of numerous suspension and adherent cells, including HeLa, BHK-21, HEK 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. This modification of MEM with Earle's Salts is supplemented with L-Glutamine. Liquid media provide basic nutrients for all cell culture applications. Each of our high-quality cell culture media is manufactured according to the originally published formula or modifications essential to the consistent performance and stability of the culture medium.