Dulbecco’s Phosphate-Buffered Saline (DPBS) is a balanced salt solution used to maintain physiological pH and osmotic balance during cell culture workflows. This sterile filtered formulation lacks calcium and magnesium, making it ideal for applications where the presence of divalent cations could interfere with enzymatic activity or cell dissociation processes.

Applications:

• Washing cells prior to dissociation with trypsin or other enzymes
• Rinsing cell monolayers during routine handling
• Preparing reagents or diluents free from calcium and magnesium
• Maintaining cell viability during short-term manipulations outside of growth medium

DPBS without Ca²⁺ and Mg²⁺ is a versatile and essential reagent for cell culture procedures requiring precise control over ionic composition.