NZY First-Strand cDNA Synthesis

128658 MB12 Media/Uploads/mb28701(22).jpg NZY First-Strand cDNA Synthesis <div class="title" style="color: rgb(0, 116, 60); background-color: rgb(255, 255, 255); padding-right: 3px; font-size: 14px; font-family: Roboto;"><span style="font-family: Tahoma; color: rgb(89, 89, 89);">Storage Conditions: -85 °C to -15 °C</span></div><p class="title" style="color: rgb(0, 116, 60); background-color: rgb(255, 255, 255); padding-right: 3px; font-size: 14px; font-family: Roboto;"><span style="color: rgb(89, 89, 89);"><span style="font-family: Tahoma;">&nbsp;</span></span></p><p class="title" style="color: rgb(0, 116, 60); background-color: rgb(255, 255, 255); padding-right: 3px; font-size: 14px; font-family: Roboto;"><span style="color: rgb(89, 89, 89);"><span style="font-family: Tahoma;"><span style="background-color: rgb(255, 255, 255); font-size: 16px; letter-spacing: -0.24px;">NZY First-Strand cDNA Synthesis Kit is a convenient and reliable system that includes all the necessary components to synthesize first-strand cDNA. The kit allows reaction at high temperatures, which are benefic when starting from difficult and complex RNA.</span></span></span></p><p class="title" style="color: rgb(0, 116, 60); background-color: rgb(255, 255, 255); padding-right: 3px; font-size: 14px; font-family: Roboto;"><span style="font-family: Tahoma;"><span style="color: rgb(89, 89, 89);"><span style="background-color: rgb(255, 255, 255); font-size: 16px; color: rgb(89, 89, 89);">NZY First-Strand cDNA Synthesis Kit is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA.The resulting single-stranded cDNA is suitable for use in real-time quantitative Reverse Transcription PCR (RT-qPCR). NZY First-Strand cDNA Synthesis Kit is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg up to 5 µg of total RNA. The kit includes a combination of random hexamers and oligo(dT)</span><span style="background-color: rgb(255, 255, 255); font-size: 8px; color: rgb(89, 89, 89);">18</span><span style="background-color: rgb(255, 255, 255); font-size: 16px; color: rgb(89, 89, 89);"> primers in order to increase sensitivity. The primers are included in the NZYRT 2× Master Mix, which also contains dNTPs, MgCl</span><span style="background-color: rgb(255, 255, 255); font-size: 8px; color: rgb(89, 89, 89);">2</span><span style="background-color: rgb(255, 255, 255); font-size: 16px; color: rgb(89, 89, 89);"> and an optimized RT buffer. NZYRT Enzyme Mix includes both the NZY Reverse Transcriptase (RNase H minus) and the NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. RNase H (from </span><i style="background-color: rgb(255, 255, 255); font-size: 16px;">E. coli</i><span style="background-color: rgb(255, 255, 255); font-size: 16px;">) is provided in a separate tube to specifically degrade the RNA template in cDNA:RNA hybrids after the first-strand cDNA synthesis. This procedure will improve the sensitivity of subsequent RT-qPCR reaction since PCR primers will bind more easily to the cDNA</span></span></span></p> 649.87 550.74 page_30315 0 0 0