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NZYTaq II 2x Green Master Mix
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Volume (ml)
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catalog number: MB35801
Volume (ml): 2 x 1.25 mL
catalog number: MB35802
Volume (ml): 4 x 1.25 mL
catalog number: MB35803
Volume (ml): 20 x 1.25 mL
price: ₪1228.66
כולל מע"מ
catalog number: MB35805
Volume (ml): 1 x 50 mL
price: ₪2081.61
כולל מע"מ
Storage Conditions: -85 °C to -15 °C
NZYTaq II 2× Green Master Mix is a premixed ready-to-use solution containing NZYTaq II DNA polymerase (MB354), which belongs to a new generation of Taq-derived DNA polymerases optimized for standard PCR applications.
NZYTaq II 2× Green Master Mix is a premixed ready-to-use solution containing NZYTaq II DNA polymerase (MB354), which belongs to a new generation of Taq-derived DNA polymerases optimized for standard PCR applications. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations and supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb. MgCl2 final concentration is 2.5 mM, allowing the implementation of a comprehensive range of PCR protocols. In addition, reactions assembled with NZYTaq II 2× Green Master Mix may be directly loaded onto agarose gels. The mix contains two dyes (blue and yellow) that allow monitoring the progress of the electrophoresis. NZYTaq II 2× Green Master Mix is not suitable when direct fluorescent or absorbance readings are required without prior purification of the amplified DNA from PCR. We recommend using the master mix version without dyes NZYTaq II 2× Colourless Master Mix (MB357) or purifying the PCR product using NZYGelpure (MB011) before performing such downstream protocols. NZYTaq II DNA polymerase lacks 3’→5’ exonuclease activity. Resulting PCR products have an A-overhang and are suitable for cloning with NZYtech’s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).
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127849
MB358
Media/Uploads/mb35801(1).jpg
NZYTaq II 2x Green Master Mix
<div class="title" style="color: rgb(0, 116, 60); background-color: rgb(255, 255, 255); padding-right: 3px; font-size: 14px; font-family: Roboto;"><span style="color: rgb(89, 89, 89);"><span style="font-family: Tahoma;">Storage Conditions: -85 °C to -15 °C</span></span></div><p class="title" style="color: rgb(0, 116, 60); background-color: rgb(255, 255, 255); padding-right: 3px; font-size: 14px; font-family: Roboto;"><span style="color: rgb(89, 89, 89);"><span style="font-family: Tahoma;"> </span></span></p><p class="title" style="color: rgb(0, 116, 60); background-color: rgb(255, 255, 255); padding-right: 3px; font-size: 14px; font-family: Roboto;"><span style="color: rgb(89, 89, 89);"><span style="font-family: Tahoma;"><span style="background-color: rgb(255, 255, 255); font-size: 16px; letter-spacing: -0.24px;">NZYTaq II 2× Green Master Mix is a premixed ready-to-use solution containing NZYTaq II DNA polymerase (MB354), which belongs to a new generation of </span><i style="color: rgb(102, 102, 102); background-color: rgb(255, 255, 255); font-size: 16px; letter-spacing: -0.24px;">Taq</i><span style="background-color: rgb(255, 255, 255); font-size: 16px; letter-spacing: -0.24px;">-derived DNA polymerases optimized for standard PCR applications.</span></span></span></p><p class="title" style="color: rgb(0, 116, 60); background-color: rgb(255, 255, 255); padding-right: 3px; font-size: 14px; font-family: Roboto;"><span style="color: rgb(89, 89, 89);"><span style="font-family: Tahoma;"><span style="background-color: rgb(255, 255, 255); font-size: 16px;">NZYTaq II 2× Green Master Mix is a premixed ready-to-use solution containing NZYTaq II DNA polymerase (MB354), which belongs to a new generation of </span><i style="background-color: rgb(255, 255, 255); font-size: 16px;">Taq</i><span style="background-color: rgb(255, 255, 255); font-size: 16px;">-derived DNA polymerases optimized for standard PCR applications. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations and supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb. MgCl</span><span style="background-color: rgb(255, 255, 255); font-size: 8px;">2</span><span style="background-color: rgb(255, 255, 255); font-size: 16px;"> final concentration is 2.5 mM, allowing the implementation of a comprehensive range of PCR protocols. In addition, reactions assembled with NZYTaq II 2× Green Master Mix may be directly loaded onto agarose gels. The mix contains two dyes (blue and yellow) that allow monitoring the progress of the electrophoresis. NZYTaq II 2× Green Master Mix is not suitable when direct fluorescent or absorbance readings are required without prior purification of the amplified DNA from PCR. We recommend using the master mix version without dyes NZYTaq II 2× Colourless Master Mix (MB357) or purifying the PCR product using NZYGelpure (MB011) before performing such downstream protocols. NZYTaq II DNA polymerase lacks 3’→5’ exonuclease activity. Resulting PCR products have an A-overhang and are suitable for cloning with NZYtech’s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).</span></span></span></p><p class="title" style="color: rgb(0, 116, 60); background-color: rgb(255, 255, 255); padding-right: 3px; font-size: 14px; font-family: Roboto;"><span style="color: rgb(89, 89, 89);"> </span></p><p class="title" style="color: rgb(0, 116, 60); background-color: rgb(255, 255, 255); padding-right: 3px; font-size: 14px; font-family: Roboto;"><span style="color: rgb(89, 89, 89);"> </span></p>
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