ATP is a marker for cell viability due to its presence in all metabolically active cells. ATP concentration declines rapidly when cells undergo necrosis or apoptosis, therefore monitoring ATP is an important indicator of cytotoxic, cytostatic and proliferative effects.
Our ATPlite 1glow, is an advanced luminescence assay that detects ATP in mammalian cells using firefly luciferase. ATP levels correlate with cell viability, making this a powerful tool for assessing cytotoxicity and proliferation.
The ATPlite 1glow reagent utilizes a homogenous "add-mix-read" format, eliminating wash and separation steps to simplify workflows in high-throughput applications. It features enhanced signal stability with a long signal half-life, offering greater flexibility for batch processing. This single reagent measures ATP changes in both 2D and 3D cell culture.
Specifications
| Application | |
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| Assay Points | 100 pts, 96-well; 400 pts, 384-well |
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| Automation Compatible | Yes |
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| Brand | |
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| Cellular or Signaling Pathway | Autophagy Cell Death Cell Growth Cytotoxicity Necrosis |
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| Detection Modality | |
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| Format | |
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| Protocol | 1-step |
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| Signal Halflife | > 3 hours |
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| Shipping Conditions | Shipped Ambient |
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| Unit Size | 10 mL |
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