ATP is a marker for cell viability due to its presence in all metabolically active cells. ATP concentration declines rapidly when cells undergo necrosis or apoptosis, and so monitoring ATP is a good indicator of cytotoxic, cytostatic and proliferation effects.
Our ATPlite 1glow F, in a ready-to-use liquid format, is an advanced luminescence assay that detects ATP in mammalian cells using firefly luciferase. As ATP levels correlate with cell viability, this assay is a powerful tool for assessing cytotoxicity and proliferation. Engineered for signal stability, this luminescence based technology provides a broad dynamic range and can reduce interference from colored compounds that may affect absorbance-based viability assays.
The ATPlite 1glow F reagent utilizes a homogenous "add-mix-read" format, eliminating wash and separation steps to simplify workflows in high-throughput applications. It features enhanced signal stability with a long signal half-life, offering greater flexibility for batch processing. This single reagent measures ATP changes in both 2D and 3D cell culture.
Specifications
| Application | |
|---|
| Assay Points | 1,000 pts, 96-well: 4,000 pts, 384-well |
|---|
| Automation Compatible | Yes |
|---|
| Brand | |
|---|
| Cellular or Signaling Pathway | Autophagy Cell Death Cell Growth Cytotoxicity Necrosis |
|---|
| Detection Modality | |
|---|
| Format | |
|---|
| Protocol | ready-to-use |
|---|
| Signal Halflife | > 3 hours |
|---|
| Shipping Conditions | Shipped in Dry Ice |
|---|
| Unit Size | 100 mL |
|---|