ATP LITE ASSAY KIT

127343 601694 Media/Uploads/6016941(1).webp ATP LITE ASSAY KIT <p style="color: rgb(0, 0, 0); background: rgb(255, 255, 255); padding: 0px; font-size: 18px; font-family: &quot;Hanken Grotesk&quot;, sans-serif; border-width: 0px; border-style: none; border-image: none;">ATP is a marker for cell viability because it is present in all metabolically active cells. Because ATP concentration declines rapidly when cells undergo necrosis or apoptosis, monitoring ATP is a good indicator of cytocidal, cytostatic and proliferation effects. Discover our ATPlite™ cell viability assay. Our ATPlite and ATPlite 1step luminescence assay systems use patented innovative technologies that measure cell viability, proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.</p><p style="color: rgb(0, 0, 0); background: rgb(255, 255, 255); padding: 0px; font-size: 18px; font-family: &quot;Hanken Grotesk&quot;, sans-serif; border-width: 0px; border-style: none; border-image: none;">ATPlite is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This luminescence cell viability assay is the alternative to colorimetric, fluorometric and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds.</p><p style="color: rgb(0, 0, 0); background: rgb(255, 255, 255); padding: 0px; font-size: 18px; font-family: &quot;Hanken Grotesk&quot;, sans-serif; border-width: 0px; border-style: none; border-image: none;">Advantages:</p><ul style="color: rgb(0, 0, 0); background: rgb(255, 255, 255); padding: 0px; font-size: 16px; font-family: &quot;Hanken Grotesk&quot;, sans-serif; border: 0px; list-style-type: none; list-style-image: initial;"><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;">Long-lived luminescent signal: half-life (t<sub style="background: transparent; padding: 0px; border: 0px;">1/2</sub>) greater than 5 hours, depending on cell type and medium</li><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;">Rapid: results generated in 15 - 25 minutes</li><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;">Simple and reproducible: no separation steps; only two reagent additions</li><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;">Homogeneous assay: no cell harvesting or centrifugation required</li><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;">Sensitive: down to 5 cells in 100µL medium (derived from CHO and HL-60 cells in 100µL medium)</li><li style="background: transparent; padding: 0px; border-top: unset; border-right: 0px; border-bottom: 0px; border-left: 0px;">Wide linear dynamic range: =10<sup style="background: transparent; padding: 0px; border: 0px;">5</sup> (as derived from CHO and HL-60 cells)</li></ul><p>&nbsp;</p><h3 class="rvty-heading-h4 overview-title" style="color: rgb(0, 0, 0); background: rgb(255, 255, 255); padding: 0px; font-size: 32px; font-family: &quot;Hanken Grotesk&quot;, sans-serif; font-weight: 500; border: 0px;">Specifications</h3><div class="panel-overview" style="color: rgb(0, 0, 0); background: rgb(255, 255, 255); padding: 0px; font-size: 16px; font-family: &quot;Hanken Grotesk&quot;, sans-serif; border-width: 0px; border-style: none; border-image: none;"><div class="specifications" style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;"></div><div class="specifications" style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;"><div class="field-group-table group_other_specifications" style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;"><div class="table-header" style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;"><label class="table-label" style="background: transparent; padding: 0px; font-size: 24px; border: 0px;">Other specifications</label></div><table class="table node-product-variation-view" style="width: 904px; padding: 0px; border: 1px solid rgb(227, 227, 227);"><tbody style="background: transparent; padding: 0px; border: 0px;"><tr class="field-application type-entity-reference table-row" style="background: transparent; width: 902px; padding: 0px; border: 0px;"><th style="background: rgb(251, 251, 251); width: 225.5px; padding: 12px; font-weight: 400; border-width: 0px 1px 0px 0px; border-style: none solid none none; border-right-color: rgb(227, 227, 227);">Application</th><td style="background: transparent; width: 676.5px; padding: 12px; border-style: none; border-width: 0px; text-align: left;"><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;"><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;">Cell Viability</div></div></td></tr><tr class="field-automation-compatible type-entity-reference table-row" style="background: transparent; width: 902px; padding: 0px; border-width: 1px 0px 0px; border-style: solid none none; border-color: rgb(227, 227, 227) currentcolor currentcolor;"><th style="background: rgb(251, 251, 251); width: 225.5px; padding: 12px; font-weight: 400; border-width: 0px 1px 0px 0px; border-style: none solid none none; border-right-color: rgb(227, 227, 227);">Automation Compatible</th><td style="background: transparent; width: 676.5px; padding: 12px; border-style: none; border-width: 0px; text-align: left;"><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;">Yes</div></td></tr><tr class="field-brand type-entity-reference table-row" style="background: transparent; width: 902px; padding: 0px; border-width: 1px 0px 0px; border-style: solid none none; border-color: rgb(227, 227, 227) currentcolor currentcolor;"><th style="background: rgb(251, 251, 251); width: 225.5px; padding: 12px; font-weight: 400; border-width: 0px 1px 0px 0px; border-style: none solid none none; border-right-color: rgb(227, 227, 227);">Brand</th><td style="background: transparent; width: 676.5px; padding: 12px; border-style: none; border-width: 0px; text-align: left;"><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;"><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;">ATPlite</div></div></td></tr><tr class="field-assay-pathway type-entity-reference table-row" style="background: transparent; width: 902px; padding: 0px; border-width: 1px 0px 0px; border-style: solid none none; border-color: rgb(227, 227, 227) currentcolor currentcolor;"><th style="background: rgb(251, 251, 251); width: 225.5px; padding: 12px; font-weight: 400; border-width: 0px 1px 0px 0px; border-style: none solid none none; border-right-color: rgb(227, 227, 227);">Cellular or Signaling Pathway</th><td style="background: transparent; width: 676.5px; padding: 12px; border-style: none; border-width: 0px; text-align: left;"><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;"><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;">Autophagy</div><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;">Cell Death</div><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;">Cell Growth</div><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;">Cytotoxicity</div><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;">Necrosis</div></div></td></tr><tr class="field-detection-method type-entity-reference table-row" style="background: transparent; width: 902px; padding: 0px; border-width: 1px 0px 0px; border-style: solid none none; border-color: rgb(227, 227, 227) currentcolor currentcolor;"><th style="background: rgb(251, 251, 251); width: 225.5px; padding: 12px; font-weight: 400; border-width: 0px 1px 0px 0px; border-style: none solid none none; border-right-color: rgb(227, 227, 227);">Detection Modality</th><td style="background: transparent; width: 676.5px; padding: 12px; border-style: none; border-width: 0px; text-align: left;"><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;"><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;">Luminescence</div></div></td></tr><tr class="field-format type-entity-reference table-row" style="background: transparent; width: 902px; padding: 0px; border-width: 1px 0px 0px; border-style: solid none none; border-color: rgb(227, 227, 227) currentcolor currentcolor;"><th style="background: rgb(251, 251, 251); width: 225.5px; padding: 12px; font-weight: 400; border-width: 0px 1px 0px 0px; border-style: none solid none none; border-right-color: rgb(227, 227, 227);">Format</th><td style="background: transparent; width: 676.5px; padding: 12px; border-style: none; border-width: 0px; text-align: left;"><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;"><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;">Microplates</div></div></td></tr><tr class="field-assay-protocol type-entity-reference table-row" style="background: transparent; width: 902px; padding: 0px; border-width: 1px 0px 0px; border-style: solid none none; border-color: rgb(227, 227, 227) currentcolor currentcolor;"><th style="background: rgb(251, 251, 251); width: 225.5px; padding: 12px; font-weight: 400; border-width: 0px 1px 0px 0px; border-style: none solid none none; border-right-color: rgb(227, 227, 227);">Protocol</th><td style="background: transparent; width: 676.5px; padding: 12px; border-style: none; border-width: 0px; text-align: left;"><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;">2-step</div></td></tr><tr class="field-signal-halflife type-string table-row" style="background: transparent; width: 902px; padding: 0px; border-width: 1px 0px 0px; border-style: solid none none; border-color: rgb(227, 227, 227) currentcolor currentcolor;"><th style="background: rgb(251, 251, 251); width: 225.5px; padding: 12px; font-weight: 400; border-width: 0px 1px 0px 0px; border-style: none solid none none; border-right-color: rgb(227, 227, 227);">Signal Halflife</th><td style="background: transparent; width: 676.5px; padding: 12px; border-style: none; border-width: 0px; text-align: left;"><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;">At least 5 hours</div></td></tr><tr class="field-shipping-conditions-descri type-entity-reference table-row" style="background: transparent; width: 902px; padding: 0px; border-width: 1px 0px 0px; border-style: solid none none; border-color: rgb(227, 227, 227) currentcolor currentcolor;"><th style="background: rgb(251, 251, 251); width: 225.5px; padding: 12px; font-weight: 400; border-width: 0px 1px 0px 0px; border-style: none solid none none; border-right-color: rgb(227, 227, 227);">Shipping Conditions</th><td style="background: transparent; width: 676.5px; padding: 12px; border-style: none; border-width: 0px; text-align: left;"><div style="background: transparent; padding: 0px; border-width: 0px; border-style: none; border-image: none;">Shipped Ambient</div></td></tr></tbody></table></div></div></div> 2405.45 2038.52 page_30269 0 0 0