MyTaq™ DNA Polymerase is recommended for all standard PCR applications. The MyTaq DNA Polymerase and MyTaq Reaction Buffer in this product, are a unique combination of next-generation polymerase and novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq has an increased affinity for DNA, enabling reliable amplification from very low amounts of template. MyTaq DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates in the presence of PCR inhibitors. The composition of the buffer system is critical for efficient PCR. MyTaq reaction buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, saving time, effort and the cost of performing unnecessary assay repeats. The combination of MyTaq and optimized buffer system allow for faster PCR reactions compared with other polymerases, therefore reducing overall run time from approximately 1 hour to under 30 minutes. This is achieved without compromising specificity or yield, reducing the reaction time allows for increased throughput and faster time to results
Publications References:
1. No evidence of human genome integration of SARS-CoV-2 found by long-read DNA sequencing
Cell Reports Methods (2021)
https://pmc.ncbi.nlm.nih.gov/articles/PMC8316065/
במאמר מצוין במפורש:
MyTaq DNA Polymerase (Bioline, BIO-21105). (PMC)
2. Development of Aptamers for RNase Inactivation in Xtract-Free™ Sample Collection and Transport Medium
Methods and Protocols (2024)
https://pmc.ncbi.nlm.nih.gov/articles/PMC11203062/
נעשה שימוש ב־MyTaq polymerase (Bioline) להגברת ספריות SELEX. (PMC)
3. Survival rate, blood feeding habits and sibling species composition of Aedes simpsoni complex
Parasites & Vectors (2022)
https://pmc.ncbi.nlm.nih.gov/articles/PMC8812930/
החוקרים השתמשו ב־MyTaq DNA Polymerase (Bioline) לצורך PCR של דגימות יתושים. (PMC)
4. Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya
PLoS Neglected Tropical Diseases (2021)
https://pmc.ncbi.nlm.nih.gov/articles/PMC7895382/
בפרק השיטות מצוין השימוש ב־MyTaq DNA Polymerase Kit (Bioline). (PMC)
5. Integrating complementary methods to improve diet analysis in fishery-targeted species
Journal of Fish Biology (2018)
https://pmc.ncbi.nlm.nih.gov/articles/PMC6194329/
נעשה שימוש ב־MyTaq DNA Polymerase (Bioline) להכנת ספריות ריצוף. (PMC)
6. Molecular diversity and frequency of the diarrheagenic enteric protozoan Giardia duodenalis and Cryptosporidium spp.
PLoS ONE (2017)
https://pmc.ncbi.nlm.nih.gov/articles/PMC5472271/
המאמר משתמש ב־MyTaq DNA Polymerase (Bioline) במספר פרוטוקולי PCR. (PMC)
7. Molecular Diagnostics of Banana Fusarium Wilt Targeting Secreted-in-Xylem Genes
Microorganisms (2019)
https://pmc.ncbi.nlm.nih.gov/articles/PMC6554419/
במאמר נעשה שימוש ב־MyTaq™ Red DNA Polymerase (Bioline). (PMC)
8. Isolation of a New Infectious Pancreatic Necrosis Virus (IPNV) Variant from a Fish Farm in Scotland
Viruses (2021)
https://pmc.ncbi.nlm.nih.gov/articles/PMC7997178/
נעשה שימוש ב־MyTaq DNA Polymerase (Bioline) להגברת הגנום הוויראלי. (PMC)