BIOTAQ™ is a purified thermostable DNA polymerase offering high yield over a wide range of PCR templates, and is a good choice for routine assays. BIOTAQ is a robust preparation and delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors. BIOTAQ is supplied with 10x NH4-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl2 is provided to allow reaction conditions to be adjusted to suit the template.
Publications References:
- An efficient method to assemble linear DNA templates for in vitro screening and selection systems
Nucleic Acids Research, 2009.
https://pmc.ncbi.nlm.nih.gov/articles/PMC2764453/
(PMC) - Development of Capsicum EST–SSR markers for species identification and in silico mapping onto the tomato genome sequence
Molecular Breeding, 2013.
https://link.springer.com/article/10.1007/s11032-012-9774-z
(Springer Nature Link) - Reverse evolution leads to genotypic incompatibility despite functional and active site convergence
eLife, 2015.
https://elifesciences.org/articles/06492
(eLife) - A non-lethal method for detection of Bonamia ostreae in flat oyster (Ostrea edulis) using environmental DNA
Scientific Reports, 2020.
https://www.nature.com/articles/s41598-020-72715-y
(Nature) - Detection and enumeration of Lak megaphages in microbiome samples by endpoint and quantitative PCR
STAR Protocols, 2022.
https://www.cell.com/star-protocols/fulltext/S2666-1667(21)01352-4
(ResearchGate) - The liver fluke Campula oblonga from harbour porpoise Phocoena phocoena stranded on the Danish North Sea Coast: updated morphometric and molecular characteristics
Parasitology Research, 2026.
https://link.springer.com/article/10.1007/s00436-026-08688-0
(Springer Nature Link) - Subcutaneous Dirofilaria repens infection in an imported dog in Denmark
Acta Veterinaria Scandinavica, 2025.
https://actavetscand.biomedcentral.com/articles/10.1186/s13028-025-00820-x
(Springer Nature Link) - Porphyromonas gingivalis Peptidylarginine Deiminase, a Key Contributor in the Pathogenesis of Experimental Periodontal Disease and Experimental Arthritis
PLOS ONE, 2014.
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0100838
(PLOS) - A Novel Connexin 30 Mutation in Clouston Syndrome
Journal of Investigative Dermatology, 2006.
https://www.sciencedirect.com/science/article/pii/S0022202X15415982
(ScienceDirect) - Investigation of genetic alterations in paediatric acute lymphoblastic leukaemia
עבודת מחקר אקדמית, University College London.
https://discovery.ucl.ac.uk/1470366/
(discovery.ucl.ac.uk)