For 24 reactions (evaluation pack): 5x ER/A-Tailing Enzyme Mix (0.24 ml) and 10x ERA Buffer (0.25 ml).
The ER/A-Tailing Enzyme Mix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
- Combines end-repair and dA-tailing into a single step — for use with fragmented DNA
- Compatible with 250 pg to 1 µg input DNA
- High library efficiency and yields
- Low duplication rates
- Uniformity across a broad range of GC content
- Compatible with input DNA containing EDTA
Product Details
ER/A Tailing Enzyme Mix is an NGS library preparation module. The module uses a one-step reaction combining end-repair and dA-tailing to convert fragmented DNA into 5ʹ-phosphorylated and 3ʹ-dA-tailed DNA fragments, enabling direct ligation of Illumina sequencing adapters. When used in combination with the WGS ligation module (cat. no. L6030-W-L), the optimized chemistry ensures high sensitivity for low input DNA, high ligation efficiency for maximum library yield and a workflow that is under 3 hours with less than 45 minutes hands on time.
ER/A Tailing Enzyme Mix is supplied with 10x ERA Buffer (cat. no B9420).
Performance
Enzyme properties
- Storage temperature: –25°C to –15°C