For 24 reactions: 5x WGS Fragmentation Mix (1 x 0.24 mL), 10X Fragmentation Buffer (1 x 0.25 mL) and Enhancer (1 x 0.25 mL)
The WGS Fragmentation Mix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
- High library yields and sensitivity
- Tunable range of fragment sizes
- Compatible with input DNA from 1 ng – 1 µg
- Reproducible libraries
- Low duplication rates and base distribution bias
- Uniformity across broad range of GC content
Product Details
WGS Fragmentation Mix provides a single-tube solution for library construction for Illumina platforms. The protocol supports fragmentation, end-repair and dA-tailing in a single reaction step, greatly simplifying the workflow and reducing the total reaction time and hands-on time.
WGS Fragmentation Mix is supplied with 10X Fragmentation Buffer (cat. no. B0330) and Enhancer (cat. no. B0340).
Performance
Genome coverage of WGS Fragmentation Mix is comparable to mechanical shearing with both 100 ng and 1 ng of DNA
WGS Fragmentation Mix provides:
- Faster workflow with high-quality, reproducible libraries
- Higher library yield and better sensitivity at 100 ng and 100 pg input
- Customizable fragment sizes and highly reproducible fragmentation
- Lower base distribution bias at starting position
- Significantly lower duplication rate for low DNA inputs
- Performance from any input
Enzyme properties :
- Storage temperature: –25°C to –15°C