50 U of E. coli Pyrophosphatase (0.5 mL at 100 U/mL)
The E. coli Pyrophosphatase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to form orthophosphate
- Removal of pyrophosphate (PPi) and its inhibitory effects enhances RNA and DNA synthesis reactions
Product Details
E. coli Pyrophosphatase catalyzes the Mg-dependent reaction of P2O7-4 + H2O → 2HPO4-2.
This enzyme is supplied in 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol.
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 19,704 Daltons
| Test | Units tested | Specification |
|---|
| Purity | n/a | >95% |
| Specific activity | n/a | 3500 U/mg |
| Single-stranded exonuclease | 35 U | <1% released |
| Double-stranded exonuclease | 35 U | <1% released |
| Double-stranded endonuclease | 35 U | No conversion |
| E. coli DNA contamination | 35 U | <10 copies |