1250 U of Thermostable Pyrophosphatase (0.625 mL at 2,000 U/mL)
The Thermostable Pyrophosphatase (1250 U) is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Hydrolysis of inorganic pyrophosphate to produce orthophosphate
- Low Km (5.4 µM)
- Active at pH 7–9
- Optimal temperature for activity at 75°C
Product Details
Thermostable pyrophosphatase is a recombinant enzyme from Sulfolobus acidocaldarius, which catalyzes the Mg2+-dependent reaction of P2O7-4 + H2O → 2HPO4-2. It has a low Km (5.4 µM) for pyrophosphate, is active between pH 7 and 9, has an optimal temperature for activity at 75°C and is functional under PCR conditions (1-3).
Supplied in:
10 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol (pH 7.5 at 25°C)
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 19.4 kDa
| Test | Amount tested | Specification |
| Purity | n/a | >95% |
| Specific activity | n/a | 3500 U/mg |
| Single-stranded exonuclease | 50 U | <1.0 % released |
| Double-stranded exonuclease | 50 U | <1.0 % released |
| Double-stranded endonuclease | 50 U | No conversion |
| E. coli DNA contamination | 50 U | <10 copies |