1.5 mg (evaluation pack) RecA (0.75 ml at 2.0 mg/ml) and 10X RecA Reaction Buffer (1.5 ml)
The RecA is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
- Promotes strand exchange of single-stranded DNA with homologous duplex DNA
- A RecA–ATP single-stranded DNA complex can function as a co-protease
- Complexed with site-specific oliognucleotides can be used to target and specifically cleave large DNA fragments
Product Details
RecA, which functions in DNA recombination and DNA repair (1, 2, 3), binds to single stranded DNA, resulting in the polymerization of RecA into a nucleoprotein complex. This complex aligns with complementary-double stranded DNA, resulting in RecA catalysis of DNA strand exchange. RecA DNA binding is stimulated by ATP hydrolysis or non-hydrolyzable ATP analogs. The RecA–ATP–single-stranded DNA complex also functions as a coprotease factor in the proteolytic cleavage of LexA, UmuD and certain bacteriophage proteins. RecA complexed with site-specific oligonucleotides has been used to target and specifically cleave large DNA fragments (4).
Supplied in:
10 mM Tris-Cl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol; pH 7.5 at 25°C.
Supplied with:
10X RecA Reaction Buffer (B9260): 700 mM Tris·Cl, 100 mM MgCl2 and 50 mM DTT; pH 7.6 at 25°C.
Performance
Enzyme properties
- Storage temperature: –25°C to –15°C
- Molecular weight: 37,973 Daltons
| Test | Amount tested | Specification |
| Purity | N/A | >95% |
| Single-stranded exonuclease | 2 µg | <5.0% released |
| Double-stranded exonuclease | 2 µg | <1.0% released |
| Double-stranded endonuclease | 2 µg | No conversion |
| E. coli DNA contamination | 5 µg | <10 copies |