The RNase H (5000 U) is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Kindly note that the delivery takes two to three weeks at the moment. We will keep you posted with any developments. Thank you for choosing us as your molecular biology partner.
Features
- Useful for removing mRNA during second-strand cDNA synthesis
- Produces ribonucleotide molecules with 5’-phosphate and 3’-hydroxyl termini
Product Details
E. coli RNase H (rnh) is an endoribonuclease that degrades the RNA strand of RNA/DNA hybrid molecules (1,2). RNase H digestion produces ribonucleotide molecules with 5’-phosphate and 3’-hydroxyl termini. RNase H is nearly inactive against single or double-stranded RNA molecules.
Supplied in:
20 mM Tris-HCl, 100 mM KCl, 0.1 mM DTT, 10 mM MgCl2, 0.1 mM EDTA, 50% glycerol (pH 7.9 at 25°C)
Supplied with:
10X RNase H Buffer (B9220): 500 mM Tris-HCl, 750 mM KCl, 30 mM MgCl2, 100 mM DTT (pH 8.3 at 25°C)
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 18.1 kDa
| Test | Amount tested | Specification |
| Purity | n/a | >99% |
| Specific activity | n/a | 625,000 U/mg |
| Single-stranded exonuclease | 500 U | <5.0 % released |
| Double-stranded exonuclease | 500 U | <1.0 % released |
| Double-stranded endonuclease | 500 U | No conversion |
| E. coli DNA contamination | 500 U | <10 copies |
| Non-specific RNase | 500 U | No detectable non-specific RNase |