10,000 U of Endonuclease VIII (1 mL of 10,000 U/mL) and 10x Endonuclease Vlll Buffer (1 x 1.5 mL).
The Endonuclease VIII is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Excises damaged pyrimidines from duplex DNA
- Cleaves AP sites leaving terminal phosphates at the 5ʹ and 3ʹ ends
Product Details
E.coli Endonuclease VIII functions as both an N-glycosylase by excising oxidative base lesions and generating an AP site, and AP-lyase, by subsequently cleaving the phosphodiester backbone and leaving terminal phosphates at the 5ʹ and 3ʹ ends (1). Damaged bases removed by Endonuclease VIII include urea, 5, 6- dihydroxythymine, thymine glycol, 5-hydroxy-5- methylhydanton, uracil glycol, 6-hydroxy-5, 6-dihydrymine and methyltartronylurea (2).
This enzyme is supplied in 10 mM Tris-HCl, 250 mM NaCl, 0.1 mM EDTA and 50% glycerol; pH 8.0 at 25°C.
At a temperature of 25°C and pH 8.0, the 10x buffer for Endonuclease Vlll comprises 100 mM Tris-HCl, 750 mM NaCl, and 10 mM EDTA.
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 29,845 Daltons