10,000 U T4 Polynucleotide Kinase (1.0 mL at 10,000 U/mL) and 10X Polynucleotide Kinase Buffer (2 x 1.5 mL)
The T4 Polynucleotide Kinase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Incorporates labeled phosphates at the 5’ end of nucleic acids
- Catalyzes transfer and exchange of phosphate to the 5′-OH of ssDNA and RNA
- Catalyzes transfer and exchange of phosphate to the 5′-OH of dsDNA
- Removes 3′ phosphoryl groups from DNA and RNA or a nucleotide/nucleoside
Product Details
T4 Polynucleotide Kinase (PNK) is widely used to phosphorylate the 5’ end of both DNA and RNA in labeling and ligation experiments. T4 PNK catalyzes the transfer and exchange of the terminal gamma position phosphate of ATP to the 5′-hydroxyl terminus of double- and single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules.
T4 PNK also catalyzes the hydrolytic removal of 3′-PO4 termini from DNA, RNA and deoxynucleoside 3′-monophosphates. The enzyme is used to remove 3′-phosphoryl groups from nucleotide/nucleoside or DNA and RNA in studies of gene expression and gene function.
In addition to its importance in molecular biology and biochemistry research, T4 PNK exemplifies a family of bifunctional enzymes with 5′-kinase and 3′-phosphatase activities that play key roles in RNA and DNA repair.
The enzyme is supplied in 10 mM Tris-HCl, 50 mM KCl, 0.1 µM ATP, 1 mM DTT, 0.1 mM EDTA, 50% glycerol; pH 7.4 at 25°C.
The enzyme is supplied with a 10X PNK Buffer (B9040) containing 700 mM Tris-HCl, 100 mM MgCl2, 50 mM DTT; pH 7.6 at 25°C.
Performance
| Test | Specification |
| Purity | >99% |
| Specific activity | 133,333 U/mg |
| Single-stranded exonuclease | 2000 U <5.0% released |
| Double-stranded exonuclease | 2000 U <1.0% released |
| Double-stranded endonuclease | 2000 U = No conversion |
| E. coli DNA contamination | 2000 U; <10 copies |