1.5 mg of E. coli Single-Stranded DNA Binding Protein (0.5 mL at 2.75 mg/mL)
The E. coli Single-Stranded DNA Binding Protein is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Binds single-stranded DNA
- Relaxes secondary DNA structure and enhances processivity in certain DNA polymerase-mediated reactions
Product Details
E. coli single-stranded DNA binding protein preferentially binds single-stranded DNA, forming a tetramer of four identical 18.9 kDa subunits, which protects 8–16 nucleotides. The protein does not bind well to double-stranded DNA. In nature, the protein participates in DNA replication, recombination and repair functions. In vitro, E. coli Single-Stranded DNA Binding Protein has been found to stimulate specific DNA polymerase-mediated reactions by relaxing DNA secondary structure and enhancing enzyme processivity.
This enzyme is supplied in 50 mM Tris-HCl, 200 mM NaCl, 1.0 mM DTT, 0.1 mM EDTA and 50% glycerol; pH 7.5 at 25°C.
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 18,975 Daltons
| Test | Units tested | Specification |
|---|
| Purity | n/a | >99% |
| Single-stranded exonuclease | 25 µg | <1% released |
| Double-stranded exonuclease | 25 µg | <1% released |
| Double-stranded endonuclease | 25 µg | No conversion |
| E. coli DNA contamination | n/a | 0.7 µg inhibits PCR |