20,000 U of RNAse Inhibitor (0.5 mL at 40,000 U/mL). Storage temperature: –25°C to –15°C
The RNase Inhibitor is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Neutralizes pancreatic-type ribonucleases e.g., RNase A, by high affinity noncovalent binding
- Does not inhibit other RNases and modifying enzymes like polymerases and reverse transcriptases
- Minimizes compromise of RNA experiments caused by the presence of RNases
- Recombinant protein expressed in E.coli carrying the porcine RNase Inhibitor gene
- Available in glycerol-free format for lyophilization
Product Details
Native ribonuclease inhibitor is a large (~450 residues, ~50 kDa), acidic (pI ~4.7), leucine-rich repeat protein. Ribonuclease inhibitor shows remarkable affinity for pancreatic-type ribonucleases such as RNase A, RNase B and RNase C and acts as a potent noncompetitive inhibitor of these enzymes. The resulting noncovalent complexes are some of the tightest known in biology. Ribonuclease inhibitor does not interfere with the activities of other RNases and modifying enzymes like polymerases and reverse transcriptases.
Ribonuclease inhibitors are crucial in molecular biology to minimize compromise of RNA experiments by the action of ribonucleases. Ribonucleases are ubiquitous and easily introduced from skin, air or labware. The primary source of RNases within most environments is microorganisms: bacteria, viruses and fungi.
Benefits of using RNase Inhibitor
- Preservation of RNA integrity by preventing degradation during handling, storage and enzymatic assays
- Enhanced experimental accuracy in sensitive applications like qPCR, RT-PCR and RNA-seq
- Improved yields in transcription/translation in in vitro transcription and translation (IVT) systems
RNase Inhibitor is recombinantly expressed in E.coli and is provided as a fusion of the porcine ribonuclease inhibitor gene with a proprietary 22.5 kDa protein tag. RNase Inhibitor is active below 50°C and under nondenaturing conditions.
Product Composition| Component | Composition | Storage temperature |
|---|
| RNase Inhibitor (Y9240) | Supplied in 20 mM Hepes-KOH, 50 mM KCl, 8 mM DTT, 50% glycerol, pH 7.5 at 25°C. | –25°C to –15°C |
This product is available in a glycerol-free (lyophilization-ready) format. Contact us for more details.
Many ribonuclease inhibitors have limited stability or activity at high temperatures restricting their use in certain applications. RNase Inhibitor Hu, a recombinant human protein, is stable up to 60°C and is suitable for use in procedures such as reverse transcription at temperatures ≥50°C. RNase Inhibitor Hu is available in pack sizes up to 1,600,000 U.