10,000 U (evaluation pack) Lambda Exonuclease (2.0 ml at 5,000 U/ml) and 10X Lambda Exo Reaction Buffer (1 x 1.5 ml).
The Lambda Exonuclease is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
- Highly processive 5ʹ→ 3ʹ exonuclease, active on blunt and 5′ recessed ends
Product Details
Lambda Exonuclease is a highly processive 5ʹ→3ʹ double-stranded exonuclease that degrades one strand of the duplex. Lambda
exonuclease can initiate a blunt DNA or DNA containing 3ʹ single-stranded overhangs. Lambda Exonuclease has greatly reduced
activity on non-phosphorylated DNA, single-stranded DNA and DNA having protruding 5ʹ single-stranded termini. Lambda
exonuclease will not initiate at a nick or gap (1,2).
Supplied in:
Lambda Exonuclease is supplied in 25 mM Tris·Cl, 50 mM NaCl, 1.0 mM DTT, 0.1 mM EDTA and 50% glycerol: pH 7.5 at 25°C.
Supplied with:
10X Lambda Exo Reaction Buffer (cat. no B8030) contains 670 mM glycine and 25 mM MgCl2, pH 9.4 at 25°C.
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 25,909 Dalton
| Test | Amount tested | Specification |
| Purity | n/a | >99% |
| Specific activity | n/a | 80,000 U/mg |
| Double-stranded endonuclease | 1500 U | No conversion |
| E. coli DNA contamination | 1500 U | <10 copies |