50,000 U Exonuclease III (100,000 U/mL) (0.5 mL) and 10X Yellow Buffer (1 x 1.5mL)
The Exonuclease III is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Exonuclease action excises bases from duplex DNA in 3’→5’ direction
- Multifunctional enzyme with phosphatase, exonuclease, AP endonuclease and RNase H activities
Product Details
Exonuclease III is a 3′→5′ exonuclease that acts by digesting one strand of a dsDNA duplex at a time or digesting the RNA strand of an RNA-DNA heteroduplex. Exonuclease III removes a limited number of nucleotides per binding event, resulting in coordinated progressive deletions within the population of DNA molecules. Exonuclease III removes 3′ terminal groups on dsDNA, efficiently degrades 3′ recessed but not 3′ protruding DNA ends (creating 5′ overhangs), breaks phosphodiester bonds on the 5′ side of AP sites in both dsDNA and ssDNA and increases turnover of the DNA glycosylase MutY.
The enzyme is supplied in 25 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol; pH 8.0 at 25°C.
The enzyme is supplied with 10X Yellow Buffer (B0130) containing 100 mM Bis-Tris-Propane-HCl, 100 mM MgCl2, 10 mM DTT; pH 7.0 at 25°C.
The 3'→5' exonuclease can be inactivated by heating at 65°C for 5 minutes.
Performance
| Assay | Specification |
| Purity | >99% |
| Specific activity | 100,000 U/mg |
| Double-stranded endonuclease | 1000 U = No conversion |
| E. coli DNA contamination | 1000 U <10 copies |
| UDG activity | <20 U/mL |