30,000 U of Exonuclease I (1.5 mL at 20,000 U/mL)
The Exonuclease I is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Excises bases on single-stranded DNA in 3ʹ→ 5ʹ direction
- Removes linear single-stranded DNA, leaving behind double-stranded DNA in the sample
Product Details
Exonuclease I cleaves single-stranded DNA in the 3ʹ→5ʹ direction, releasing 5ʹ mono/di-nucleotides and leaving double-stranded DNA molecules and the 5ʹ terminus intact. The enzyme is processive though digestion is inhibited by a 3ʹ terminal phosphate. Exonuclease I is tolerant of a wide range of buffer conditions and can typically be added to reactions containing magnesium (1–3).
This enzyme is supplied in 10 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.5 mM EDTA and 50% glycerol: pH 7.5 at 25°C.
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 54,500 Daltons
| Test | Units tested | Specification |
|---|
| Purity | n/a | >99% |
| Specific activity | n/a | 185,000 U/mg |
| Double-stranded endonuclease | 200 U | No conversion |
| E. coli DNA contamination | 200 U | <10 copies |