TaqNova HS DNA Polymerase, 10x TaqNova HS Reaction Buffer, 50 mM MgCl2 Storage temperature: -20°C
The TaqNova HS DNA Polymerase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Catalyzes DNA synthesis in a 5’ → 3’ direction
- Shows no 3′ → 5’ exonuclease activity but has a 5’ → 3’ exonuclease activity
- Enables easy setup of a hot-start PCR reaction at room temperature
- Minimizes amplification of non-specific products and primer-dimers
- Amplifies fragments of up to 5 kb and leaves ´A´ overhangs
Product Details
TaqNova HS DNA Polymerase is a mixture of of recombinant thermostable Taq DNA polymerase from Thermus aquaticus and a highly specific monoclonal antibody, which acts as an inhibitor of the polymerization activity. The TaqNovaHS enables easy setup of a hot-start PCR reaction at room temperature.
It is recommended for a wide range of demanding applications that require highly specific amplification. The TaqNovaHS polymerase is a universal and easy-to-use DNA polymerase that works rapidly and effectively in various PCR conditions. The enzyme catalyzes DNA synthesis in a 5’ → 3’ direction, shows no 3′ → 5’ exonuclease activity, but has a 5’ → 3’ exonuclease activity.
It is supplied with 20 mM Tris-HCl (pH 8.0, 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.
One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μL reaction.
Performance
| Assay | Specification |
| Functional activity | Passed |
| DNase contamination | None detected |