ZipScript 1-Step RT-qPCR Mix (1000 rxn)

The ZipScript One-Step RT-qPCR Kit and ZipScript WarmX One-Step RT-qPCR Kit are highly sensitive and reproducible RT-qPCR solutions optimized for real-time PCR. The high-efficiency RNA-dependent multiplex qPCR formulation enables a broad range of sample detection. ZipScript features a blend of RNase H minus reverse transcriptase (EnzScript Reverse Transcriptase) and a fast-activating hot start DNA polymerase (Phoenix Hot Start Taq DNA Polymerase). For operational convenience, detect as little as 0.1 pg of RNA in a four plex assay with the one-step reaction format. In addition to market-leading RT-qPCR performance, world-class manufacturing by QIAGEN ensures consistent, repeatable results in your laboratory.

The ZipScript WarmX One-Step RT-qPCR Kit features an aptamer based warm start capacity that blocks reverse transcription during reaction setup at room temperature, improving assay specificity and consistency. Reactions can be set up at room temperature with more confidence, especially when using high-throughput automated solutions.

Supplied with: 
2X ZipScript Reaction Buffer 1 (B7641L)
Inquire for lyophilized or lyo-ready options.

ZipScript One-Step RT-qPCR Mix is a blend of EnzScript Reverse Transcriptase and Phoenix Hot Start Taq DNA Polymerase enzymes. EnzScript is a robust, RNA-dependent DNA polymerase with no detectable RNase H activity. The enzyme can be used to generate first-strand cDNA from mRNA or total RNA within an optimal temperature range between 42–50ºC. 

The Phoenix Polymerase is a recombinant, thermostable Taq DNA polymerase complexed with a thermolabile, neutralizing antibody. This fast-activating antibody-mediated hot-start capability enhances the overall specificity, sensitivity, and yield of the PCR reaction by reducing nonspecific amplification and primer-dimer formation prior to cycling. Together with EnzScript, Phoenix creates ZipScript, an extremely sensitive RT-qPCR mix with: 

• Broad detection range: High sensitivity (<0.1pg total human RNA) combined with a broad range of detection (>7 orders of 
  magnitude).
• Superior multiplexing: Multiplex up to 4 targets per reaction.
• Market-leading performance: Superior sensitivity, specificity, reproducibility and efficiency compared to existing industry-leading formulations.
• Reliable throughput: Ideal for high-throughput environments where ease of use, automation compatibility, and result 
  accuracy are critical.
• Backed by quality: QIAGEN quality ensures lot-to-lot consistency, reducing inter-assay variability and maximizing data 
  validity.

The aptamer based warm start reverse transcriptase in the 10X ZipScript WarmX One-Step RT-qPCR Mix reduces non-specific reverse transcription during reaction setup at room temperature and improves assay specificity and consistency. The addition of the WarmX feature facilitates high-throughput automation solutions. 

ZipScript WarmX:

• Reduces non-specific and spurious reverse transcription during reaction setup at room temperature.
• Maintains robust assay performance after extended (up to 24 hours) preincubation at room temperature.
• Prevents loss of detection or lower amplitude resulting from room temperature preincubation.

Mix properties

• Storage temperature: –25°C to –15°C