5000 U of TaqIT DNA Polymerase (50,000 U/mL) and 10x TaqIT Reaction Buffer.
The TaqIT DNA Polymerase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Exonuclease-deficient derivative of Taq DNA Polymerase
- More thermostable and greater fidelity than full-length Taq DNA Polymerase
Product Details
TaqIT DNA Polymerase is an exonuclease-deficient derivative of Taq DNA polymerase.
The enzyme is supplied in 20 mM Tris-HCl, 222 mM (NH4)2SO4, 10 mM β-mercaptoethanol, 0.1 mM EDTA, 0.1% Brij 58 and 50% glycerol; pH 8.6 at 25°C.
The 10x TaqIT Reaction Buffer contains 500 mM Tris-HCl, 160 mM (NH4)2SO4, 35 mM MgCl2 and 0.25% Brij 58; pH 9.2 at 25⁰C.
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 62,407 Daltons
| Test | Units tested | Specification |
|---|
| Purity | n/a | >99% |
| Specific activity | n/a | 42,000 U/mg |
| Single-stranded exonuclease | 500 | <1% released |
| Double-stranded exonuclease | 500 | <1% released |
| Double-stranded endonuclease | 500 | No conversion |
| E. coli DNA contamination | 500 | <10 copies |