250 reactions (evaluation pack) of 2x VeraSeq PCR Mix (1 x 6.25 mL)
The VeraSeq PCR Mix (2x, 250 reactions) is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Certain applications in which this product can be used may be covered by patents issued and applicable in the United States and abroad. Purchase of this product does not include a license to perform any patented application; therefore, it is the sole responsibility of the users of the product to determine whether they may be required to engage a license agreement depending upon the particular application in which the product is used.
Features
- 2x master mix based on VeraSeq™ 2.0 (cat. no. P7511L)
- 50x greater fidelity than Taq DNA Polymerase
- Ultra-thermostable polymerase
- Extends 1 kb in 15 seconds
- Strong proofreading activity (3'→5' exonuclease activity)
Product Details
VeraSeq™ PCR Mix is a premixed, ready-to-use 2x solution containing VeraSeq™ 2.0 High-Fidelity DNA Polymerase (P7511L), dNTPS, MgCl2 and reaction buffer at optimal concentrations to maximize the speed, accuracy and length of DNA synthesis. The formulation provides efficient, high-fidelity DNA amplification, cloning and synthetic biology applications.
Performance
Polymerase properties
- Storage temperature: –25°C to –15°C
- Extension rate: 15 second per kb at 72˚C
- Proofreading (3'→5' exo): Yes, strong
- Nick-translation (5'→3' exo): No
- Fidelity: >50x higher than Taq DNA Polymerase
- Strand displacement: No
- Thermostability: Highly thermostable
- Able to extend an RNA primer: No
- Extends from a nick: No
- Generate blunt end products: Yes
- Uracil read through: No
| Test | Specification |
| Functional Assay | Amplification of 500 bp fragment from genomic DNA |
Principle
Source of recombinant enzyme protein
The protein is produced by a recombinant E. coli strain carrying the engineered VeraSeq™ 2.0 gene.
Unit definition
One unit is defined as the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble form at 74°C in 30 minutes.
Procedure
Protocol
General precautions should be taken when setting up a PCR, including setting up the reaction on ice, adding master mix last, gently pipetting, thorough mixing and a quick centrifugation. The following procedure can be used as a guideline. Reactions may need to be optimized individually.
Reaction setup (for 50 µL)
| Component | Volume (µL) | Final concentration |
| Sterile H2O | 20, variable | N/A |
| 2x VeraSeq PCR Mix | 25 | 1x |
| PCR Primer Cocktail | 5 | 0.5 µM each |
| Library DNA* | Variable | N/A |