500 U of Phoenix Hot Start Taq DNA Polymerase (0.10mL at 5,000 U/mL), 5x Phoenix Hot Start Taq Reaction Buffer (4 x 1.5 mL), and 5x Phoenix Hot Start Taq GC Reaction Buffer (2 x 1.5 mL)
The Phoenix Hot Start Taq DNA Polymerase is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
- Thermostable 5ʹ→3ʹ DNA polymerase with antibody-mediated hot start
- Increased specificity, sensitivity and yield in PCR reactions
- Lacks a 3ʹ→5ʹ proofreading function but retains 5ʹ→3ʹ exonuclease activity
- 72-hour stability at room temperature for simple setup and automated workflows
- Tolerance to wide ranges of Mg2+ and annealing temperatures
Product Details
Phoenix™ Hot Start Taq DNA Polymerase provides antibody-based hot start for robust PCR performance with exceptional pre-PCR cycling room-temperature stability.
Supplied in:
20 mM Tris-HCl, 100 mM NaCl, 0.1 mM EDTA, Stabilizer and 50% glycerol; pH 7.5 at 25°C.
Supplied with:
5X Phoenix Hot Start Taq Reaction Buffer (B7590) and 5X Phoenix Hot Start Taq GC Reaction Buffer (B7591)
OEM by QIAGEN offers bulk manufacturing of Phoenix Hot Start Taq DNA Polymerase in custom formulations, including Low
glycerol and glycerol-free formulations.
Performance
Phoenix Hot Start Taq DNA Polymerase is a recombinant, thermostable Taq DNA polymerase complexed with a thermostable, neutralizing antibody that blocks the 5ʹ→3ʹ polymerase activity prior to the initial DNA denaturation step of PCR (1, 2). Such antibody-mediated hot-start capability enhances the overall specificity, sensitivity and yield of the PCR by reducing nonspecific amplification and primer–dimer formation prior to PCR cycling and allows the convenience of reaction set up at room temperature. When the temperature of the PCR mixture reaches ≥94°C during the initial DNA denaturing step of PCR cycling, activity of the Taq DNA polymerase is fully restored. Phoenix Hot Start Taq DNA Polymerase, like standard Taq DNA polymerase, also has 5ʹ→3ʹ exonuclease activity, but lacks any detectable 3ʹ→5ʹ exonuclease activity.
- Increased amplification specificity compared to regular Taq DNA polymerase
- Increased amplification yield and overall success rate
- Rapid reactivation and shorter PCR cycle time with antibody method
- High sensitivity amplification with 300 pg human genomic DNA
- Multiplexing capability with minimal optimization
- High success rates with GC-rich targets
Polymerase properties
Storage temperature: –25°C to –15°C
| Test | Units Tested | Specification |
| Purity | N/A | |
| Specific activity | N/A | 74,625 U/mg |
| Single-stranded exonuclease | 50 U | <10% |
| Double-stranded exonuclease | 50 U | <1% |
| Double-stranded endonuclease | 50 U | No conversion |
| Taq inhibition | N/A | Pass |
| Functional assay | N/A | Functional with buffers ordered with cat. nos. B7590 and B759 |