500 U of VeraSeq ULtra DNA Polymerase, 5X VeraSeq Buffer II and 5X VeraSeq GC Buffer.
The VeraSeq™ ULtra DNA Polymerase is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
- Read and use uracil residue with maximum speed and accuracy
- Ultra-thermostable polymerase
- 25x greater fidelity than Taq DNA polymerase
- Functions in Mg2+ concentrations 1.5 to 3.0 mM
- Strong proofreading function (3'-5' exonuclease)
- OEM by QIAGEN offers bulk manufacturing of VeraSeq ULtra DNA Polymerase in custom formulations, including low-glycerol or hot-start formulations
Product Details
VeraSeq™ ULtra DNA Polymerase is an engineered, ultra-thermostable, uracil-literate polymerase designed to maximize the speed, accuracy, and length of DNA synthesis during sequencing template preparation. The result is a novel enzyme that can read through uracil, extend a kilobase of sequence in 15 seconds and has an accuracy 25 times higher than Taq DNA Polymerase.
Supplied in:
20 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, stabilizer and 50% glycerol; pH 7.4 at 25°C.
Supplied with:
5X VeraSeq™ Buffer II (B7102L) and 5X VeraSeq™ GC Buffer (B7130L).
You can ask us about low-glycerol or hot-start formulations.
Performance
Polymerase properties
Extension rate: 15 seconds per kilobase at 72˚C
Proofreading (3'-5' exo): Yes, strong
Nick-translation (5'-3' exo): No
Fidelity: > 25X higher than Taq DNA Polymerase
Strand displacement: No
Thermostability: Highly thermostable
Able to extend an RNA primer: No
Extends from a nick: No
Generate blunt end products: Yes
Uracil read through: Yes
Storage temperature: –25°C to –15°C
| Test | Units tested | Specification |
| Purity | n/a | > 95% |
| Specific activity | n/a | 100,000 U/mg |
| Double-stranded endonuclease | 120 U | No conversion |
| E. coli DNA contamination | 150 U | <10 copies |