500 U of VeraSeq 2.0 High-Fidelity DNA Polymerase (0.25 mL at 2,000 U/mL) with 5x VeraSeq Buffer II (6 x 1.5 mL) and 5x VeraSeq GC Buffer (3 x 1.5 mL)
The VeraSeq™ 2.0 High-Fidelity DNA Polymerase is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
- 50x greater fidelity than Taq DNA Polymerase
- Extends 1 kb in 15 seconds
- Strong proofreading activity (3'→5' exonuclease activity)
- Functions in Mg2+ concentrations 1.5 to 3.0 mM
- QIAGEN Strategic Partnership & OEM offers customized bulk manufacturing of this enzyme, including low glycerol and hot
start formulations
Product Details
VeraSeq™ 2.0 High-Fidelity DNA Polymerase is an engineered, ultra-thermostable polymerase that delivers industry-leading
speed, fidelity and robustness to PCR amplification. The novel enzyme can extend a kilobase of sequence in 15 seconds and with
an accuracy 50 times higher than Taq DNA Polymerase.
Supplied in:
20 mM Tris·HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, Stabilizer and 50% glycerol; pH 7.4 at 25°C.
Supplied with:
5x VeraSeq™ Buffer II (B7102L) and 5x VeraSeq™ GC Buffer (B7130L).
You can also ask us about low glycerol or hot-start formulations.
Performance
Polymerase properties
Extension rate: 15 seconds per kb at 72˚C
Proofreading (3'→5' exo): Yes, strong
Nick-translation (5'→3' exo): No
Fidelity: >50x higher than Taq DNA Polymerase
Strand displacement: No
Thermostability: Highly thermostable
Able to extend an RNA primer: No
Extends from a nick: No
Generate blunt-end products: Yes
Uracil read through: No
- Storage temperature: –25°C to –15°C
| Test | Units Tested | Specification |
| Purity | n/a | >95% |
| Specific activity | n/a | 100,000 U/mg |
| Double-stranded endonuclease | 120 U | No conversion |
| E. coli DNA contamination | 150 U | <10 copies |