1000 U of Poly(A) Polymerase (5000 U/mL) and 10x Poly(A) Polymerase Reaction Buffer.
The Poly(A) Polymerase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Catalyzes the addition of AMP from ATP to the 3’ hydroxyl of RNA
Product Details
Poly(A) Polymerase attaches ATP (catalyzed from AMP) to the 3ʹ hydroxyl of RNA, which is useful for making poly(A)-tailed RNA.
The enzyme is supplied in 25 mM Tris-HCl, 500 mM NaCl, 1 mM MgCl2, 0.1 mM DTT, 0.1 mM EDTA and 50% glycerol; pH 8.0 at 25°C.
The reaction buffer for Poly(A) Polymerase at 10x concentration includes 500 mM Tris-HCl, 2.5 M NaCl, and 100 mM MgCl2, with a pH of 7.9 at 25°C.
Performance
Poly(A) Polymerase catalyzes the addition of AMP from ATP to the 3ʹ hydroxyl of RNA. The reaction requires Mg2+ and is template independent.
- Storage temperature: –25°C to –15°C
- Molecular weight: 53,870 Daltons
| Test | Units tested | Specification |
|---|
| Purity | | >95% |
| Specific activity | | >20,000 U/mg |
| Single-stranded exonuclease | 200 U | <5% released |
| Double-stranded exonuclease | 200 U | <1% released |
| Double-stranded endonuclease | 200 U | No conversion |
| E. coli DNA contamination | 100 U | <10 copies |
| RNase contamination | 200 U | No detectable non-specific RNase |