Bst X DNA Polymerase (40000 U)

Bst X DNA Polymerase is a recombinant, thermostable DNA polymerase from Geobacillus that is homologous to the well-known DNA polymerase from Bacillus stearothermophilus (Bst). The Bst X enzyme is similar to Bst polymerase in that it lacks 5'→3' and 3'→5' exonuclease activity and exhibits strong strand displacement activity allowing it to perform well in LAMP isothermal amplification technologies. Relative to Bst polymerase, however, Bst X displays improved amplification speed, salt tolerance, and reaction sensitivity.

Bst X DNA Polymerase is supplied in 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, <0.1% Triton X-100, 50% glycerol and 10 mM Tris-HCl; pH 7.5 at 25°C.

Bst X DNA Polymerase is provided with 10x Xcelerator Reaction Buffer (B7390) and 100 mM Magnesium Sulfate Solution.

10x Bst X Xcelerator Reaction Buffer (B7390) contains 2 mM MgSO₄ at 1x concentration. Depending on the application, it may be necessary to adjust the MgSO₄ from a final concentration of 2 mM to a 10 mM.

Ask about low glycerol formulations

Properties

• Optimal reaction temperature from 60-70°C.
• Heat inactivated at 80°C after 10 min incubation.
• High tolerance to the non-ionic detergents Tween-20, Tween-80, Triton X-100, and NP-40 (up to 5% final concentration).
• Active in salt from 40 to 125 mM.
  • Alternate temperature
  Approximately 15% activity at 37°C.
  Approximately 5% activity at 25°C

• Storage temperature: –25°C to –15°C
• Molecular weight: 66.5 kDa