3500 U of T7 DNA Polymerase (0.35 mL at 10,000 U/mL) and 10X T7 DNA Polymerase Buffer (1 x 1.5 mL)
The T7 DNA Polymerase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Powerful 3′→5′ exonuclease activity that acts on both single and double-stranded DNA
- Mesophilic, highly processive, replicative DNA Polymerase
Product Details
T7 DNA Polymerase is the mesophilic, highly processive, replicative DNA polymerase from bacteriophage T7 that is responsible for the rapid and accurate replication of the virus’ genome during its infection cycle. T7 DNA Polymerase is a two-subunit protein, consisting of a polymerase domain (gene 5 from the T7 bacteriophage) and a processivity factor (E. coli trxA gene thioredoxin) (1,2). The enzyme possesses a powerful (3′→5′) exonuclease activity that acts on both single and double stranded DNA and appears to be responsible for the high fidelity of this enzyme and prevents strand displacement synthesis (3,4,5).
T7 DNA Polymerase is a highly processive DNA polymerase with an exceptionally high synthesis and replication fidelity rate. The enzyme is supplied in 50 mM KPO4, 0.1 mM EDTA, 1.0 mM DTT and 50% glycerol; pH 7.0 at 25°C.
10X T7 DNA Polymerase Buffer (B7260) contains 400 mM Tris-HCl, 200 mM MgCl2 and 500 mM NaCl; pH 7.5 at 25°C.
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 92,140 Daltons
| Test | Specification |
|---|
| Purity | >99% |
| Specific activity | 13,333 U/mg |
| Single-stranded exonuclease | Functional |
| Double-stranded exonuclease | Functional |
| Double-stranded endonuclease | 100 U, no conversion |
| E. coli DNA contamination | 100 U, <10 copies |