50,000 U/mL of T7 RNA Polymerase and 10X T7 RNA Polymerase Buffer
The T7 RNA Polymerase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- DNA-dependent RNA polymerase
- High specificity for the T7 promoter
- Catalyzes the Mg2+ dependent synthesis of RNA from rNTPs
Product Details
T7 RNA Polymerase provides 5ʹ→ 3ʹ RNA synthesis of RNA from a DNA template. T7 RNA polymerase requires its T7 double-stranded DNA promoter to initiate transcription, but it can transcribe RNA from both single-stranded and double-stranded DNA templates. The enzyme is supplied in 50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol and <0.1% Triton X-100; pH 7.9 at 25°C.
10X T7 RNA Polymerase Buffer (B7180) contains 400 mM Tris-HCl, 60 mM MgCl2, 100 mM DTT and 20 mM spermidine; pH 7.9 at 25°C.
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 98,855 Daltons
| Test | Units tested | Specification |
|---|
| Purity | n/a | >99% |
| Specific activity | n/a | 312,500 U/mg |
| Single-stranded exonuclease | 500 U | <1% released |
| Double-stranded exonuclease | 500 U | <1% released |
| Double-stranded endonuclease | 500 U | No conversion |
| E. coli DNA contamination | 500 U | <10 copies |
| RNAse DNA contamination | 500 U | No detectable non-specific RNAse |