100,000 U (evaluation pack) of Manta 1.0 DNA Polymerase (0.25 mL at 400,000 U/mL) and 10x PCR Buffer II (5 x 1.5 mL)
The Manta 1.0 DNA Polymerase (high concentration) is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
- Thermostable DNA polymerase (Bst)
- Strong strand-displacement activity
- No 5'→3' exonuclease activity and 3'→5' exonuclease activity
Product Details
Manta 1.0 DNA Polymerase is a Thermostable bacillus (Bst) DNA polymerase that exhibits strong strand displacement. This polymerase is deficient in both proofreading (3ʹ→ 5ʹ) and nick-translation (5ʹ→ 3ʹ) nuclease activities. The protein was originally characterized and its crystal structure solved by Lorena Beese (1).
Supplied in:
10 mM Tris-HCl, 50 mM KCl, 1.0 mM DTT, 0.1 mM EDTA, <0.1% Triton X-100 and 50% glycerol; pH 7.5 at 25°C.
Supplied with:
10X PCR Buffer II (cat. no. B7140) contains 200 mM Tris-HCl, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, and 1.0%
Triton X-100; pH 8.8 at 25°C.
Ask about low glycerol formulations.
Performance
Polymerase properties
- Storage temperature: –25°C to –15°C
| Test | Units Tested | Specification |
| Purity | n/a | >99% |
| Specific activity | n/a | 400,000 U/mg |
| Single-stranded exonuclease | 4000 U | <5.0% released |
| Double-stranded exonuclease | 4000 U | <1.0% released |
| Double-stranded endonuclease | 4000 U | no conversion |
| E. coli DNA contamination | 4000 U | <10 copies |