2000 U of T4 DNA Polymerase (3000 U/mL) and 10x Blue Buffer. Storage temperature: –25°C to –15°C
The T4 DNA Polymerase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Exhibits a polymerase activity, which catalyzes DNA synthesis from the 5' to 3' end
- Exhibits a powerful 3′→5′ exonuclease activity, the so-called proofreading function
- Lacks 5′→3′ exonuclease or strand displacement functions
Product Details
T4 DNA Polymerase catalyzes the extension of a primed DNA template in the 5′→3′ direction. This enzyme exhibits a powerful 3′→5′ exonuclease activity, while lacking any 5′→3′ exonuclease or strand displacement functions.
The protein is produced by a recombinant E. coli strain carrying the T4 DNA Polymerase gene.
The enzyme is supplied in 100 mM KPO4,1mM DTT, 0.1mM EDTA, 50% glycerol pH 6.5 at 25⁰C.
It is supplied with a 10X Blue Buffer (B0110) containing: 500mM NaCl, 100mM Tris-HCl, 100mM MgCl2, 10mM DTT, pH 7.9 at 25⁰C.
Performance
| Assay | Specification |
| Purity | >99% |
| Specific activity | 5555 U/mg |
| Single-stranded exonuclease | Functional |
| Double-stranded exonuclease | Functional |
| Double-stranded endonuclease | 30 U; no conversion |
| E. coli DNA contamination | 30 U; <10 copies |