6000 U of Terminal Deoxynucleotidyl Transferase (TdT) (0.30 mL at 20,000 U/mL) and 10x Green Buffer (1 x 1.5 mL) and 2.5 mM Cobalt Chloride (1 x 1.5 mL)
The Terminal Deoxynulceotidyl Transferase (TdT) (6000 U) is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Efficiently extends blunt, 5’ overhanging, single-stranded DNA and RNA
- Useful for 3’ labeling
- Incorporates dATP and dTTP with a fivefold higher efficiency than dCTP and dGTP
Product Details
Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3’ hydroxyl terminus of single or double-stranded DNA molecules. The presence of 1 mM Co2+ stimulates the tailing of the 3’-ends of DNA fragments (1,2).
Supplied in:
50 mM KPO4, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol (pH 7.3 at 25°C)
Supplied with:
10X Green Buffer (B0120): 200 mM Tris-Acetate, 500 mM Potassium Acetate, 100 mM Magnesium Acetate (pH 7.9 at 25°C)
B0220: 2.5 mM CoCl2
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 82.6 kDa
| Test | Amount tested | Specification |
| Purity | n/a | >99% |
| Specific activity | n/a | 27,400 U/mg |
| Single-stranded exonuclease | 200 U | <5.0 % released |
| Double-stranded exonuclease | 200 U | <1.0 % released |
| Double-stranded endonuclease | 200 U | No conversion |
| E. coli DNA contamination | 200 U | <10 copies |