2500 U of Klenow Fragment (0.5 mL at 5000 U/mL) and 10x Blue Buffer (1 x 1.5 mL)
The Klenow Fragment is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Mesophilic DNA polymerase
- No 5'➞3' exonuclease activity
- Retains 3'➞5' exonuclease activity displaying moderate strand displacement
Product Details
Klenow Fragment is a mesophilic DNA polymerase derived from the E. coli Polymerase I DNA-dependent repair enzyme. The enzyme exhibits DNA synthesis and proofreading (3ʹ→5ʹ) nuclease activities and, in the absence of the holoenzyme’s (5ʹ→3ʹ) nuclease domain, displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated E. coli PolA gene product.
The enzyme is supplied in 100 mM KPO4, 1.0 mM DTT, 0.1mM EDTA and 50% glycerol; pH 7.4 at 25°C.
The buffer solution known as 10x Blue Buffer has a pH of 7.9 at 25°C and includes 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl2 and 10 mM DTT.
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 68,202 Daltons
| Test | Specification |
|---|
| Purity | >99% |
| Specific activity | 5000 U/mg |
| Single-stranded exonuclease | 50 U; functional |
| Double-stranded exonuclease | 50 U; functional |
| Double-stranded endonuclease | 50 U; no conversion |
| E. coli DNA contamination | 50 U; <10 copies |