2,000 U of Phi29 DNA Polymerase (0.20 mL at 10,000 U/mL) and 10x Phi29 DNA Polymerase Reaction Buffer (1 x 1.5 mL)
The Phi29 DNA Polymerase is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
- Highly processive DNA polymerase
- Powerful strand displacement activity
- 3'→5' exonuclease activity (proofreading)
Product Details
Phi29 DNA Polymerase is responsible for the replication of the Bacillus subtilis phage Phi29 (1). The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity (2) and a high fidelity 3ʹ→5ʹ proofreading exonuclease function (3), making it ideal for isothermal amplification.
The enzyme is supplied in 10 mM Tris-HCl, 100 mM KCl, 0,1 mM EDTA, 1 mM DTT, 0.5% Tween-20, 0.5% NP-40 and 50% glycerol; pH 7.4 at 25°C,
10X Phi29 DNA Polymerase Reaction Buffer (cat. no. B7020) contains the following: 500 mM Tris-HClm 100 mM (NH4)2SO4, 40 mM DTT and 100 mM MgCl2; pH 7.5 at 25°C.
Ask about REACH compliant formulations.
Performance
Properties
- Storage temperature: –25°C to –15°C
- Exonuclease activity: 3'→5'
- Molecular weight: 66,713 Daltons
| Test | Specification |
| Purity | >99% |
| Specific activity | 83,333 U/mg |
| Single-stranded exonuclease | Functional |
| Double-stranded endonuclease | 100 U: no conversion |
| E. coli DNA contamination | 100 U; 83,333 U/mg |