10,000 U of Klenow (3'→5' exo–) (0.2 mL at 50,000 U/mL) and 10X Blue Buffer (2 x 1.5 mL)
The Klenow Fragment (3'→5' exo–) is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- DNA polymerase lacking proofreading (3'→5') and nick-translation (3'→5') activities
- Retains robust 3'→5' DNA polymerase activity
- Used in fluorescent labeling reactions for microarray
- Best for dA and dT tailing for adapter ligation to DNA fragments for next-gen sequencing
Product Details
Klenow Fragment (3'→5' exo-) is the large fragment of DNA polymerase I (Klenow Fragment) that has been rendered deficient in both proofreading (3'→5') and nick-translation (3'→5') nuclease activities. Klenow Fragment (3'→5' exo-) is a mesophilic DNA polymerase that displays a moderate strand displacement activity during DNA synthesis.
The enzyme is supplied in 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol pH 7.5 @ 25⁰C.
The enzyme is supplied with 10X Blue Buffer (B0110) containing 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl2, 10 mM DTT; pH 7.9 at 25°C.
Performance
| Assay | Specification |
| Purity | >99% |
| Specific activity | 10,000 U/mg |
| Single-stranded exonuclease | 500 U <10% released |
| Double-stranded exonuclease | 500 U <1% released |
| Double-stranded endonuclease | 500 U = No conversion |
| E. coli DNA contamination | 500 U <10 copies |
| UDG activity | <20 U/mL |